Anti-KIF14抗体(ab3747)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF14抗体
参阅全部 KIF14 一抗 -
描述
兔多克隆抗体to KIF14 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.
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阳性对照
- HeLa whole cell extract.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 190 kDa. | |
| IP | Use at 1-4 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/1000. |
靶标
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功能
Plays an essential role in cytokinesis. -
序列相似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
发展阶段
Up-regulated in cells progressing through G2/M phase. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
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数据库链接
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
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别名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
图片
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Western blot - Anti-KIF14 antibody (ab3747)Sample: HeLa whole cell lysate
1) WB - 50
µ g2) IP - 1mg
Antibody: Affinity purified Rabbit anti-KIF (ab3747)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
µ g / mg of lysate.Detection: ECL.
Sample: HeLa whole cell lysate
1) WB - 50
µg 2) IP - 1mg
Antibody: Affinity purified Rabbit anti-KIF (ab3747)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
µg / mg of lysate. Detection: ECL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF14 antibody (ab3747)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-KIF14 antibody (ab3747)Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 - Input.
Lane 2 - IP.
Lane 3 - Control IgG IP.
文献 (1)
ab3747 被引用在 1 文献中.
- Schiewek J et al. Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J Cancer Res Clin Oncol 144:2195-2205 (2018). PubMed: 30094535
