Anti-KIF14抗体(ab3747)
Key features and details
- Rabbit polyclonal to KIF14
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIF14抗体
参阅全部 KIF14 一抗 -
描述
兔多克隆抗体to KIF14 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.
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阳性对照
- HeLa whole cell extract.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 190 kDa. | |
| IP | Use at 1-4 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Plays an essential role in cytokinesis. -
序列相似性
Belongs to the kinesin-like protein family.
Contains 1 FHA domain.
Contains 1 kinesin-motor domain. -
发展阶段
Up-regulated in cells progressing through G2/M phase. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring. - Information by UniProt
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数据库链接
- Entrez Gene: 9928 Human
- Omim: 611279 Human
- SwissProt: Q15058 Human
- Unigene: 3104 Human
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别名
- KIAA0042 antibody
- KIF 14 antibody
- KIF14 antibody
see all
图片
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Western blot - Anti-KIF14 antibody (ab3747)
Sample: HeLa whole cell lysate
1) WB - 50
µ g2) IP - 1mg
Antibody: Affinity purified Rabbit anti-KIF (ab3747)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
µ g / mg of lysate.Detection: ECL.
Sample: HeLa whole cell lysate
1) WB - 50
µg 2) IP - 1mg
Antibody: Affinity purified Rabbit anti-KIF (ab3747)
1) & 2) WB - 1 / 4000 dilution.
2) IP - concentration of 3
µg / mg of lysate. Detection: ECL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF14 antibody (ab3747)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-KIF14 antibody (ab3747)Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Lane 1 - Input.
Lane 2 - IP.
Lane 3 - Control IgG IP.
实验方案
文献 (1)
ab3747 被引用在 1 文献中.
- Schiewek J et al. Clinical relevance of cytoskeleton associated proteins for ovarian cancer. J Cancer Res Clin Oncol 144:2195-2205 (2018). PubMed: 30094535
