Anti-KIAA0101抗体(ab226255)
Key features and details
- Rabbit polyclonal to KIAA0101
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIAA0101抗体 -
描述
兔多克隆抗体to KIAA0101 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human KIAA0101 aa 1-50. The exact sequence is proprietary. (NP_055551.1).
Database link: Q15004 -
阳性对照
- IP: HeLa whole cell lysate (ab150035). IHC-P: Human prostate carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab226255 was affinity purified using an epitope specific to PAF immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in protection of cells from UV-induced cell death. -
组织特异性
Expressed predominantly in liver, pancreas and placenta. Not detected in heart or brain. Highly expressed in a number of tumors, especially esophageal tumors, in anaplastic thyroid carcinomas and in non-small-cell lung cancer lines. -
细胞定位
Nucleus. Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 9768 Human
- Omim: 610696 Human
- SwissProt: Q15004 Human
- Unigene: 81892 Human
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别名
- HCV NS5A transactivated protein 9 antibody
- HCV NS5A-transactivated protein 9 antibody
- Hepatitis C virus NS5A transactivated protein 9 antibody
see all
图片
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KIAA0101 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226255 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226255 at 1 µg/ml.
Lane 1: ab226255 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KIAA0101 using ab226255 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab226255 被引用在 6 文献中.
- Sun Z et al. Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR-383-5p and regulating KIAA0101. Thorac Cancer 13:1751-1762 (2022). PubMed: 35524161
- Kim MJ et al. PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis. Mol Cell 81:1698-1714.e6 (2021). PubMed: 33626321
- Li M et al. PAF enhances cancer stem cell properties via ß-catenin signaling in hepatocellular carcinoma. Cell Cycle 20:1010-1020 (2021). PubMed: 33970778
- Zhang Y et al. Circular RNA TADA2A promotes proliferation and migration via modulating of miR-638/KIAA0101 signal in non-small cell lung cancer. Oncol Rep 46:N/A (2021). PubMed: 34296306
- Pan L et al. Curcumin inhibits prostate cancer progression by regulating the miR-30a-5p/PCLAF axis. Exp Ther Med 22:969 (2021). PubMed: 34335911
- Lv W et al. KIAA0101 inhibition suppresses cell proliferation and cell cycle progression by promoting the interaction between p53 and Sp1 in breast cancer. Biochem Biophys Res Commun 503:600-606 (2018). PubMed: 29902451