Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Breast cancer)
Permeabilization
Yes - with Triton X-100 1%
Specification
Breast cancer
Blocking step
Reagent as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + BSA 1% + Tween-20 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: ab236466
Host species: Mouse
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Mouse
Clonality: Polyclonal
Conjugation: HRP polymer
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Lucille Cartier
Verified customer
提交于 Jun 29 2021