Anti-Ki67抗体[MKI67/2465] - BSA and Azide free (ab237864)
Key features and details
- Mouse monoclonal [MKI67/2465] to Ki67 - BSA and Azide free
- Suitable for: ICC, Protein Array, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
概述
-
产品名称
Anti-Ki67抗体[MKI67/2465] - BSA and Azide free
参阅全部 Ki67 一抗 -
描述
小鼠单克隆抗体[MKI67/2465] to Ki67 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: ICC, Protein Array, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Ki67 aa 2293-2478. The exact sequence is proprietary.
Database link: P46013 -
阳性对照
- IHC-P: Human tonsil tissue. ICC: MCF7 cells. Flow cyt: HeLa cells.
-
常规说明
ab237864 is the carrier-free version of ab238020.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
-
纯度
Protein A/G purified -
纯化说明
Purified from bioreactor concentrate. -
克隆
单克隆 -
克隆编号
MKI67/2465 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab237864于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use a concentration of 1 - 2 µg/ml.
|
|
Protein Array |
Use at an assay dependent concentration.
|
|
IHC-P |
Use a concentration of 1 - 2 µg/ml.
Primary incubation for 30 minutes at room temperature. |
|
Flow Cyt |
Use 1-2µg for 106 cells.
Trypsinize (0.25%) cell for 2-4 minutes. Fix with 2-4 % PFA. |
说明 |
---|
ICC
Use a concentration of 1 - 2 µg/ml. |
Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Primary incubation for 30 minutes at room temperature. |
Flow Cyt
Use 1-2µg for 106 cells. Trypsinize (0.25%) cell for 2-4 minutes. Fix with 2-4 % PFA. |
靶标
-
功能
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. -
序列相似性
Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain. -
发展阶段
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815). -
翻译后修饰
Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. -
细胞定位
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). - Information by UniProt
-
数据库链接
- Entrez Gene: 4288 Human
- Omim: 176741 Human
- SwissProt: P46013 Human
- Unigene: 689823 Human
- Unigene: 80976 Human
-
别名
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
see all
图片
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Ki67 with ab238020 at 2 μg per 106 cells (blue) compared to an isotype control (red). A Goat Anti-mouse CF488 secondary antibody was used.
This data was produced with ab238020, the same antibody in a different formulation with BSA and Azide.
-
This data was produced with ab238020, the same antibody in a different formulation with BSA and Azide.
ab238020 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab237864 尚未被引用在任何文献中。