存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/400. PubMed: 18635871|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. We recommend using an ABC system for detection of this antibody.|
|IHC-Fr||1/25 - 1/50. 1/25 - 1/50. We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein.|
功能Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
序列相似性Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain.
发展阶段Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
翻译后修饰Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
细胞定位Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
- Information by UniProt
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody ab833 at 5 ug/mL for 30 min at RT.
Immunohistochemical analysis of Human skin tissue, staining Ki67 (red) with ab833.
Antigen retrieval was performed by heat mediation in a EDTA/Tris buffer (pH 8.0). Sections were blocked with 10% NGS for 1 hour at room temperature, followed by incubation with primary antibody (1/25). An AlexFluor®546 anti-rabbit IgG (1/500) was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Ki67 with ab833 at 1/50 dilution.
HeLa cells are the classic example of an immortalized human epithelial cell line from a fatal cervical carcinoma transformed by human papillomavirus 18 (HPV18) that grown as adherent cells layer and maintain contact inhibition in vitro. 2% paraformaldehyde for 15 min was used to fix the cells, and permealized the cells with Triton-X100 (0.1%) for 10 min and then incubated cells with primary antibody Ki-67 (1/50) for overnight at 4 degree centigrade. Image was taken using a confocal laser scanning microscope that equipped with an additional differential Interference Contrast (DIC) mode. Image here demonstrate DAPI (blue-nuclear stain, upper left panel), Ki-67 (Green, upper right panel, shows nuclear localization in actively dividing cells), Hela cells in culture-DIC (phase) image (lower left panel) and superimpose image (lower right panel). The expression of the human Ki-67 protein is strictly associated with cell proliferation. During interphase, the antigen is exclusively detected as several prominent specks within the nucleus and a weak finely dispersed staining throughout the nucleoplasm, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. The fact that the Ki-67 protein is present during all active phases of the cell cycle [G(1), S, G(2), and mitosis], but is absent from resting cells [G(0)], makes it an excellent marker for determining the so-called Ki-67 staining index (Ki67-SI) of a given cell population.
ab833 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with Ki-67, using ABC and AEC chromogen.
This product has been referenced in:
- Kang MH et al. Estrogen-related receptor gamma functions as a tumor suppressor in gastric cancer. Nat Commun 9:1920 (2018). Read more (PubMed: 29765046) »
- Zonneville J et al. TGF-ß signaling promotes tumor vasculature by enhancing the pericyte-endothelium association. BMC Cancer 18:670 (2018). Read more (PubMed: 29921235) »