Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Small intestine (jejunum))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 2M HCl (60C) for 8min
Permeabilization
Yes - 0.25% Triton X-100
Specification
Small intestine (jejunum)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBST
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Egi Kardia
Verified customer
提交于 Apr 17 2020