产品名称Anti-KDM3B / JMJD1B抗体
参阅全部 KDM3B / JMJD1B 一抗
描述兔多克隆抗体to KDM3B / JMJD1B
经测试应用适用于: IHC-P, WB, IPmore details
预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
Database link: Q7LBC6
- HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab70797 was affinity purified using an epitope specific to KDM3B / JMJD1B immobilized on solid support.
Our Abpromise guarantee covers the use of ab70797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IF||1/50 - 1/250.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
功能Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
组织特异性Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
序列相似性Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain.
结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
- Information by UniProt
- 5qNCA antibody
- C5orf7 antibody
- JHDM2B antibody
All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 191 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 30 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Zepp M et al. IDK1 is a rat monoclonal antibody against hypoglycosylated bone sialoprotein with application as biomarker and therapeutic agent in breast cancer skeletal metastasis. J Pathol Clin Res 4:55-68 (2018). Read more (PubMed: 29416877) »
- Liu Y et al. An epigenetic role for PRL-3 as a regulator of H3K9 methylation in colorectal cancer. Gut : (2012). Human . Read more (PubMed: 22345654) »