重组Anti-KDEL抗体[EPR12668] (ab176333)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12668] to KDEL
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-KDEL抗体[EPR12668]
参阅全部 KDEL 一抗 -
描述
兔单克隆抗体[EPR12668] to KDEL -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293T, MCF-7, Raji, HepG2, HeLa, Jurkat, and K562 cell lysates. MCF7 and HeLa cells. Paraffin-embedded Human heart and kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.06% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12668 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-KDEL antibody [EPR12668] - ER Marker (ab184819)
- Alexa Fluor® 594 Anti-KDEL antibody [EPR12668] - ER Marker (ab203411)
- Alexa Fluor® 555 Anti-KDEL antibody [EPR12668] - ER Marker (ab203420)
- Alexa Fluor® 647 Anti-KDEL antibody [EPR12668] - ER Marker (ab214714)
- Anti-KDEL antibody [EPR12668] - BSA and Azide free (ab240190)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176333于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
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IHC-P |
1/50 - 1/350. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/250.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
IHC-P
1/50 - 1/350. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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相关性
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. -
细胞定位
Endoplasmic reticulum -
别名
- KDEL antibody
- Lys Asp Glu Leu antibody
图片
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Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling KDEL with purified ab176333 at 1/200. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).
For negative control 1, ab150120 (Rabbit primary antibody) and anti-mouse secondary antibody were used and for negative control 2, ab7291 (Mouse primary antibody) and ab150077 (anti-rabbit secondary antibody) were used.
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All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa -
Lane 1 : Anti-P4HB antibody [EPR9498] (ab137119) at 1/5000 dilution
Lanes 2 & 4 & 6 & 8 & 10 : Anti-KDEL antibody [EPR12668] (ab176333) at 1/5000 dilution
Lane 3 : Anti-GRP78 BiP antibody [EPR4040(2)] (ab108613) at 1/5000 dilution
Lane 5 : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/5000 dilution
Lane 7 : Anti-ERp57 antibody [EPR10678(B)] (ab154191) at 1/5000 dilution
Lane 9 : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/5000 dilution
Lane 11 : Anti-LEPRE1/P3H1 antibody [EPR10193(B)] (ab154799) at 1/5000 dilution
All lanes : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 25 kDa
Exposure time: 3 minutesab176333 detected several bands which have similar MWs with some proteins containing carboxyl terminal KDEL motifs, including P4HB, GRP78 BiP, GRP94, PDIA6 and LEPRE1 (PMID: 25683117, PMID: 19741001, PMID: 22079671, PMID: 28648146, PMID: 22615817). It indicates that ab176333 might recognize these proteins.
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Immunohistochemical analysis of paraffin-embedded human liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Overlay histogram showing 4% paraformaldehyde fixed HeLa cells labelling KDEL (red) with purified ab176333 at dilution of 1/100. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG at dilution of 1/2000. A non-specific IgG antibody (rabbit monoclonal) was used as isotype control (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
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Immunohistochemical analysis of paraffin-embedded rat kidney sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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All lanes : Anti-KDEL antibody [EPR12668] (ab176333) at 1/10000 dilution
Lane 1 : Raw264.7 whole cell lysate
Lane 2 : C6 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 25 kDaObserved band size : 94,78,57,48
Blocking/Diluting buffer 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded mouse liver sections labelling KDEL with purified ab176333 at dilution of 1:350. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab176333 被引用在 16 文献中.
- Malik A et al. Interleukin-10 Mitigates Doxorubicin-Induced Endoplasmic Reticulum Stress as Well as Cardiomyopathy. Biomedicines 10:N/A (2022). PubMed: 35453640
- Yan X et al. Myocilin Gene Mutation Induced Autophagy Activation Causes Dysfunction of Trabecular Meshwork Cells. Front Cell Dev Biol 10:900777 (2022). PubMed: 35615698
- Wu Q et al. Zonisamide alleviates cardiac hypertrophy in rats by increasing Hrd1 expression and inhibiting endoplasmic reticulum stress. Acta Pharmacol Sin 42:1587-1597 (2021). PubMed: 33495518
- Afanasyeva TAV et al. A look into retinal organoids: methods, analytical techniques, and applications. Cell Mol Life Sci 78:6505-6532 (2021). PubMed: 34420069
- Calì T & Brini M Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells. Nat Protoc 16:5287-5308 (2021). PubMed: 34686857