Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS Cells)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
Transfected with HA-Tip60 or GFP
Specification
U2OS Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 70 kDa
Additional data
Additional Notes
Note the band detected was from a fusion protein with HA- tag. The molecular weight of endogenous band should be determined experimentally with siRNA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 06 2017