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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-KAT13A / SRC1抗体(ab2859)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (6)

Product price, shipping and contact information

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Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
  • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
  • Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)

Key features and details

  • Rabbit polyclonal to KAT13A / SRC1
  • Suitable for: ICC, IHC-Fr, Flow Cyt
  • Reacts with: Rat, Human
  • Isotype: IgG

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Recombinant Human KAT13A / SRC1 protein (ab81791)

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概述

  • 产品名称

    Anti-KAT13A / SRC1抗体
    参阅全部 KAT13A / SRC1 一抗
  • 描述

    兔多克隆抗体to KAT13A / SRC1
  • 宿主

    Rabbit
  • 特异性

    Detects steroid receptor coactivator-1 (SRC-1).
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-Fr
    Rat
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 8-24.
    Sequence:

    SSDPANPDSHKRKGSPC


    Database link: Q15788
    (Peptide available as ab5848)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human KAT13A / SRC1 peptide (ab5848)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human KAT13A / SRC1 protein (ab81791)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC
Human
IHC-Fr
Rat
All applications
Pig
应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
IHC-Fr (1)
Use a concentration of 1 µg/ml.
Flow Cyt
Use 3-5µg for 106 cells.
说明
ICC
Use at an assay dependent concentration.
IHC-Fr
Use a concentration of 1 µg/ml.
Flow Cyt
Use 3-5µg for 106 cells.

靶标

  • 功能

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
  • 组织特异性

    Widely expressed.
  • 疾病相关

    Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
  • 序列相似性

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域

    The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
    Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
  • 翻译后修饰

    Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
    Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession Q15788 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8648 Human
    • Entrez Gene: 574068 Pig
    • Entrez Gene: 313929 Rat
    • Omim: 602691 Human
    • SwissProt: Q15788 Human
    • SwissProt: Q4PJW2 Pig
    • Unigene: 596314 Human
    • Unigene: 165307 Rat
    • 别名

      • bHLHe74 antibody
      • Class E basic helix-loop-helix protein 74 antibody
      • F SRC 1 antibody
      • Hin 2 protein antibody
      • Hin2 protein antibody
      • MGC129719 antibody
      • MGC129720 antibody
      • mNRC 1 antibody
      • NCoA 1 antibody
      • NCoA-1 antibody
      • Ncoa1 antibody
      • NCOA1_HUMAN antibody
      • Nuclear receptor coactivator 1 antibody
      • Nuclear receptor coactivator protein 1 antibody
      • NY REN 52 antigen antibody
      • Protein Hin 2 antibody
      • Protein Hin-2 antibody
      • Protein Hin2 antibody
      • Renal carcinoma antigen NY REN 52 antibody
      • Renal carcinoma antigen NY-REN-52 antibody
      • RIP 160 antibody
      • RIP160 antibody
      • SRC 1 antibody
      • SRC-1 antibody
      • Steroid receptor coactivator 1 antibody
      see all

    图片

    • Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
      Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)

      Immunolocalization of SRC1 in HeLa cells using ab2859.

    • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
      Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
      ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
      Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)

      Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.

    • Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)
      Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)

      Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.

    实验方案

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (6)

    发表研究结果有使用 ab2859?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2859 被引用在 6 文献中.

    • Castellaro AM  et al. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 30736340
    • Kong X  et al. Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression. Mol Metab 29:1-11 (2019). PubMed: 31668381
    • Kong X  et al. FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic ß-Cells. J Diabetes Res 2019:8915818 (2019). PubMed: 31485455
    • Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582
    • Hurtado A  et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . PubMed: 19005469
    • Yasumoto H  et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . PubMed: 17623774

    客户评价及客户问答

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    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7 Cells)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    30 µg
    Treatment
    Vehicle, 10nm estrogen, and 1uM tamoxifen
    Specification
    MCF7 Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 23 2019

    Question

    Inquiry: Dear Sir/Madam I would like to have the price and the delivery time

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2012

    Answer

    Thank you for contacting us.

    I am not sure if you intend to send this email to Abcam however we can offer the following products

    ab84; https://www.abcam.com/KAT13A-SRC1-antibody-1135-H4-ChIP-Grade-ab84.html

    ab2859; https://www.abcam.com/KAT13A-SRC1-antibody-ChIP-Grade-ab2859.html

    ab10308; https://www.abcam.com/KAT13A-SRC1-antibody-ab10308.html

    ab71531; https://www.abcam.com/KAT13A-SRC1-antibody-Aminoterminal-end-ab71531.html

    ab5407; https://www.abcam.com/KAT13A-SRC1-antibody-ab5407.html

    ab67101; https://www.abcam.com/KAT13A-SRC1-antibody-3532C6a-ab67101.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Apr 23 2012

    Western blot abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (huh-7 cells)
    Loading amount
    10000 cells
    Specification
    huh-7 cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 11 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% TritonX-100 in PBS for 10 min at 4'C
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 37°C
    Read More

    Dr. Sungsoo Lee

    Verified customer

    提交于 May 19 2008

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Dr. Jiqiang Zhang

    Verified customer

    提交于 Jun 20 2007

    Immunohistochemistry (Frozen sections) abreview for Anti-KAT13A / SRC1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1%
    Read More

    Dr. Jiqiang Zhang

    Verified customer

    提交于 May 23 2007

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