Anti-KAT13A / SRC1抗体(ab2859)
Key features and details
- Rabbit polyclonal to KAT13A / SRC1
- Suitable for: ICC, IHC-Fr, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-KAT13A / SRC1抗体
参阅全部 KAT13A / SRC1 一抗 -
描述
兔多克隆抗体to KAT13A / SRC1 -
宿主
Rabbit -
特异性
Detects steroid receptor coactivator-1 (SRC-1). -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIHC-Fr Rat
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免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2859 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| IHC-Fr |
Rat
|
| All applications |
Pig
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
|
| Flow Cyt |
Use 3-5µg for 106 cells.
|
| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IHC-Fr
Use a concentration of 1 µg/ml. |
|
Flow Cyt
Use 3-5µg for 106 cells. |
靶标
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功能
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. -
组织特异性
Widely expressed. -
疾病相关
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children. -
序列相似性
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
结构域
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro. -
翻译后修饰
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8648 Human
- Entrez Gene: 574068 Pig
- Entrez Gene: 313929 Rat
- Omim: 602691 Human
- SwissProt: Q15788 Human
- SwissProt: Q4PJW2 Pig
- Unigene: 596314 Human
- Unigene: 165307 Rat
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别名
- bHLHe74 antibody
- Class E basic helix-loop-helix protein 74 antibody
- F SRC 1 antibody
see all
图片
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Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
Immunolocalization of SRC1 in HeLa cells using ab2859.
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Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhangab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary. -
Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.
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Flow Cytometry - Anti-KAT13A / SRC1 antibody (ab2859)Flow cytometry analysis of SRC1 was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SRC1 Rabbit Polyclonal Antibody (PA1840, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2859 被引用在 6 文献中.
- Castellaro AM et al. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 30736340
- Kong X et al. Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression. Mol Metab 29:1-11 (2019). PubMed: 31668381
- Kong X et al. FXR Mediates Adenylyl Cyclase 8 Expression in Pancreatic ß-Cells. J Diabetes Res 2019:8915818 (2019). PubMed: 31485455
- Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582
- Hurtado A et al. Regulation of ERBB2 by oestrogen receptor-PAX2 determines response to tamoxifen. Nature 456:663-6 (2008). WB, ChIP ; Human . PubMed: 19005469
- Yasumoto H et al. GNL3L inhibits activity of estrogen-related receptor gamma by competing for coactivator binding. J Cell Sci 120:2532-43 (2007). WB . PubMed: 17623774