Key features and details
- Mouse monoclonal [4G7] to Kappa light chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
产品名称Anti-Kappa light chain抗体[4G7]
参阅全部 Kappa light chain 一抗
描述小鼠单克隆抗体[4G7] to Kappa light chain
特异性Specific for independent constantly expressed epitope of kappa chain. Reacts with free and heavy chain-associated kappa chains.
Tested Applications & Species
Application Species IHC-PHuman
Full length protein corresponding to Human Kappa light chain.
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
Concentration varies from lot to lot and can be provided on request.
This product was changed from ascites to tissue culture supernatant in March 2015. Lot numbers higher than GR145316-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Tissue culture supernatant
纯化说明Purity tested by electrophoresis.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
相关性Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
细胞定位Cytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
IHC image of Kappa light chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1936, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab1936 被引用在 2 文献中.
- De Franceschi N et al. Selective integrin endocytosis is driven by interactions between the integrin a-chain and AP2. Nat Struct Mol Biol 23:172-9 (2016). PubMed: 26779610
- Rintanen N et al. Calpains promote a2ß1 integrin turnover in nonrecycling integrin pathway. Mol Biol Cell 23:448-63 (2012). PubMed: 22160595