Anti-KANK2抗体(ab99351)
Key features and details
- Rabbit polyclonal to KANK2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KANK2抗体
参阅全部 KANK2 一抗 -
描述
兔多克隆抗体to KANK2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human KANK2 aa 550-650.
Database link: Q63ZY3 -
阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: PBS, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99351于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
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| IP |
Use a concentration of 2 - 5 µg/ml.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa. |
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IP
Use a concentration of 2 - 5 µg/ml. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
KANK2 contains 5 ANK repeats. It is strongly expressed in cervix, colon, heart, kidney and lung. -
细胞定位
Cytoplasm. Mitochondrion. -
数据库链接
- Entrez Gene: 25959 Human
- Omim: 614610 Human
- SwissProt: Q63ZY3 Human
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别名
- ANKRD25 antibody
- Ankyrin repeat domain 25 antibody
- Ankyrin repeat domain containing protein 25 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KANK2 antibody (ab99351)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KANK2 with ab99351 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-KANK2 antibody (ab99351)All lanes : Anti-KANK2 antibody (ab99351) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-KANK2 antibody (ab99351)Detection of Human KANK2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). ab99351 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate (lane 1). KANK2 was also immunoprecipitated by another rabbit anti-KANK2 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 30 seconds.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab99351 尚未被引用在任何文献中。
