Anti-Kallikrein 11抗体(ab233278)
Key features and details
- Rabbit polyclonal to Kallikrein 11
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Kallikrein 11抗体
参阅全部 Kallikrein 11 一抗 -
描述
兔多克隆抗体to Kallikrein 11 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human Kallikrein 11 aa 1-300. Expressed in E.coli. N-terminal tags.
Database link: Q9UBX7 -
阳性对照
- IHC-P: Human prostate, prostate cancer, kidney and stomach cancer tissues. WB: Mouse thymus and skin lysates; K562 cell lysate; Recombinant human Kallikrein 11 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
ab233278 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 31 kDa.
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说明 |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 31 kDa. |
靶标
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功能
Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. -
组织特异性
Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level). -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻译后修饰
About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11012 Human
- Entrez Gene: 56538 Mouse
- Omim: 604434 Human
- SwissProt: Q9UBX7 Human
- SwissProt: Q9QYN3 Mouse
- Unigene: 57771 Human
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别名
- Hippostasin antibody
- hK11 antibody
- Kallikrein 11 antibody
see all
图片
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All lanes : Anti-Kallikrein 11 antibody (ab233278) at 2 µg/ml
Lane 1 : Mouse thymus lysate
Lane 2 : Mouse skin lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa -
Paraffin-embedded human prostate tissue stained for Kallikrein 11 using ab233278 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human prostate cancer tissue stained for Kallikrein 11 using ab233278 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human kidney tissue stained for Kallikrein 11 using ab233278 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Paraffin-embedded human stomach cancer tissue stained for Kallikrein 11 using ab233278 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 µg/ml. DAB staining.
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Anti-Kallikrein 11 antibody (ab233278) at 2 µg/ml + Recombinant human Kallikrein 11 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 31 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab233278 尚未被引用在任何文献中。