重组Anti-K Cadherin/CDH6抗体[EPR7394] (ab133632)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7394] to K Cadherin/CDH6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-K Cadherin/CDH6抗体[EPR7394]
参阅全部 K Cadherin/CDH6 一抗 -
描述
兔单克隆抗体[EPR7394] to K Cadherin/CDH6 -
宿主
Rabbit -
特异性
The immunogen used for this product shares very high homology with family members Cadherin-9, Cadherin-2, Cadherin-4, Cadherin-7, Cadherin-8, Cadherin-11. Cross-reactivity with these proteins has not been confirmed experimentally.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human K Cadherin/CDH6 aa 750-850. The exact sequence is proprietary.
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阳性对照
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 1.18 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR7394 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133632于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa). |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
组织特异性
Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas. -
序列相似性
Contains 5 cadherin domains. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1004 Human
- Entrez Gene: 12563 Mouse
- Entrez Gene: 25409 Rat
- Omim: 603007 Human
- SwissProt: P55285 Human
- SwissProt: P97326 Mouse
- SwissProt: P55280 Rat
- Unigene: 171054 Human
see all -
别名
- CAD6 antibody
- CADH6_HUMAN antibody
- Cadherin 6 antibody
see all
图片
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All lanes : Anti-K Cadherin/CDH6 antibody [EPR7394] (ab133632) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Fetal brain lysate
Lane 4 : A549 cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : 293T cell lysate
Lane 7 : Fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 88 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133632 被引用在 4 文献中.
- Guagliardo NA et al. Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes. Nat Commun 11:1679 (2020). PubMed: 32245948
- Park SR et al. Tryptophanyl-tRNA Synthetase, a Novel Damage-Induced Cytokine, Significantly Increases the Therapeutic Effects of Endometrial Stem Cells. Mol Ther 28:2458-2472 (2020). PubMed: 32592690
- Hiraga K et al. Redundant type II cadherins define neuroepithelial cell states for cytoarchitectonic robustness. Commun Biol 3:574 (2020). PubMed: 33060832
- Ren T et al. CD49b inhibits osteogenic differentiation and plays an important role in osteosarcoma progression. Oncotarget 8:87848-87859 (2017). PubMed: 29152125