概述

  • 产品名称
    Anti-K Cadherin抗体
    参阅全部 K Cadherin 一抗
  • 描述
    兔多克隆抗体to K Cadherin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, Flow Cyt, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the C-terminal region of human K Cadherin.

  • 阳性对照
    • 293 cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab64917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/50 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).

In recent lot testing (Dec 2013), the apparent MW (130 kDa) is higher than the predicted MW (88 kDa) in over expression lysate, human brain and U251 cells under reduced and denatured conditions.

ELISA 1/1000.

靶标

  • 功能
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • 组织特异性
    Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas.
  • 序列相似性
    Contains 5 cadherin domains.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAD6 antibody
    • CADH6_HUMAN antibody
    • Cadherin 6 antibody
    • Cadherin 6 type 2 antibody
    • Cadherin 6 type 2 K cadherin (fetal kidney) antibody
    • Cadherin fetal kidney antibody
    • Cadherin-6 antibody
    • Cadherin6 antibody
    • CDH 6 antibody
    • Cdh6 antibody
    • K-cadherin antibody
    • KCAD antibody
    • Kidney cadherin antibody
    see all

图片

  • Anti-K Cadherin antibody (ab64917) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 88 kDa
    Observed band size: 88 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Flow cytometry analysis of NCI-H292 cells labeling K Cadherin with ab64917. A FITC-conjugated Goat anti-rabbit was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocarcinoma tissue sections labeling K Cadherin with ab64917.

文献

This product has been referenced in:
  • Webber HC  et al. The Role of Wnt/ß-Catenin Signaling and K-Cadherin in the Regulation of Intraocular Pressure. Invest Ophthalmol Vis Sci 59:1454-1466 (2018). WB, ICC/IF ; Human . Read more (PubMed: 29625468) »
  • Ma C  et al. Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma. Oncol Lett 15:9498-9506 (2018). Read more (PubMed: 29805672) »
See all 5 Publications for this product

客户评价及客户问答

1-5 of 5 Q&A

Answer

Human K Cadherin full length protein (ab132981) refers to the isoform 2 of the protein (Uniprot ID P55285) which is composed of 633 amino acids. This isoform lacks amino acids 664-790 that can be found on the isoform 1.


Anti-K Cadherin antibody, ab64917 will recognize only the isoform 1 (Uniprot ID P55285) of human K Cadherin (CDH6) from immunogen sequence analysis because this immunogen is synthetic peptide between 665-695 amino acids from the C-terminal region of human K Cadherin (CDH6).

Hence ab64917 cannot be used to detect ab132981 and unfortunately, we do not have any Human K Cadherin full length protein constituting of amino acids 1-790 (isoform 1).

We do however have the following antibodies which would recognise this protein (isoform2):

Anti-K Cadherin antibody (ab68342)
Anti-K Cadherin antibody - N-terminal (ab71434)

If you would prefer to purchase a K Cadherin isoform 1 protein rather than using one of the above antibodies, I would recommend that you look at the following website which has an excellent life science search facility which includes many suppliers: http://www.biocompare.com

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Answer

The antibody is indeed ab64917 which is now added in waiting for stock order 1184710.

Please let me know if you have any other question

Have a nice day!

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1182977.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

PS: Please note this product is currently out of stock.

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Answer

My colleague is out of office today I am sorry to hear that your customer is experiencing difficulties using the antibody.

The batch/lot has been used in a publication with IF data:


Montserrat N et al. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. J Biol Chem. 2012 Jul 13;287(29):24131-8.

If the customer has an overexpressed lysate as control, it might help with setting up the Western blot conditions. Alternatively,ab64917 recognize the same target on the C-term.

I hope this is helpful and please let me know if you need further assistance

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Answer

Thank you for contacting us.

The immunogen peptide islocated between aa.

As you seem to be quite happy about the antibody's performance in WB and hopefully soon in ELISA, maybe I could encourage you to write an Abreview to share your experienceso that future customers can make an informed decision about the usefulness of a particular product for their specific experiments?

To find out more about our Abreview system, please see the following webpage:

https://www.abcam.com/abreviews


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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