存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液Preservative: 0.09% Sodium azide
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纯度Immunogen affinity purified
纯化说明ab64917 is purified through a protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab64917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/50 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 88 kDa).
In recent lot testing (Dec 2013), the apparent MW (130 kDa) is higher than the predicted MW (88 kDa) in over expression lysate, human brain and U251 cells under reduced and denatured conditions.
功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
组织特异性Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas.
序列相似性Contains 5 cadherin domains.
- Information by UniProt
- CAD6 antibody
- CADH6_HUMAN antibody
- Cadherin 6 antibody
Anti-K Cadherin antibody (ab64917) at 1/60 dilution + 293 cell line lysates at 35 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Flow cytometry analysis of NCI-H292 cells labeling K Cadherin with ab64917. A FITC-conjugated Goat anti-rabbit was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocarcinoma tissue sections labeling K Cadherin with ab64917.
This product has been referenced in:
- Webber HC et al. The Role of Wnt/ß-Catenin Signaling and K-Cadherin in the Regulation of Intraocular Pressure. Invest Ophthalmol Vis Sci 59:1454-1466 (2018). WB, ICC/IF ; Human . Read more (PubMed: 29625468) »
- Ma C et al. Combined overexpression of cadherin 6, cadherin 11 and cluster of differentiation 44 is associated with lymph node metastasis and poor prognosis in oral squamous cell carcinoma. Oncol Lett 15:9498-9506 (2018). Read more (PubMed: 29805672) »