为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Cardiovascular Angiogenesis Inhibitors

Anti-Jagged1抗体(ab7771)

  • Datasheet
  • SDS
Reviews (14)Q&A (10)References (70)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
  • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
  • Western blot - Anti-Jagged1 antibody (ab7771)

Key features and details

  • Rabbit polyclonal to Jagged1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)
一抗
Product image
Anti-NOTCH4 antibody [EPNCIR101B] (ab166605)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-Jagged1抗体
    参阅全部 Jagged1 一抗
  • 描述

    兔多克隆抗体to Jagged1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Mouse liver lysate. IHC: Human cervical and human cervical cancer tissues. ICC/IF: Human corneal epithelial cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Inhibitors
    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7771于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (2)
Use at an assay dependent concentration.
IHC-P (4)
1/100.
WB (2)
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate.
说明
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/100.
WB
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate.

靶标

  • 功能

    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
  • 组织特异性

    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
  • 疾病相关

    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.
  • 序列相似性

    Contains 1 DSL domain.
    Contains 15 EGF-like domains.
  • 发展阶段

    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession P78504 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 182 Human
    • Entrez Gene: 16449 Mouse
    • Entrez Gene: 29146 Rat
    • Omim: 601920 Human
    • SwissProt: P78504 Human
    • SwissProt: Q9QXX0 Mouse
    • SwissProt: Q63722 Rat
    • Unigene: 224012 Human
    • Unigene: 22398 Mouse
    • Unigene: 88804 Rat
    see all
  • 别名

    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast Laboratory

    Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin

  • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)
    Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)This image is courtesy of Aihua Ma, Univdersity of Cardiff

    Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast Laboratory
    Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
  • Western blot - Anti-Jagged1 antibody (ab7771)
    Western blot - Anti-Jagged1 antibody (ab7771)

    ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
    Exposure time: 1 minute. Expected molecular weight: 134 kDa

    Lanes 1 to 3 and 5: 20µg of cell lysate per lane
    Lane 4: 20 µl of 1x LDS loading buffer

    Lane 1: Human Brain tissue lysate
    Lane 2: Human Kidney tissue Lysate
    Lane 3: Human liver tissue lysate
    Lane 4: 1x LDS loading buffer
    Lane 5: Mouse liver lysate

     

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (70)

发表研究结果有使用 ab7771?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7771 被引用在 70 文献中.

  • Masoudi M  et al. Notch signaling pathway in cumulus cells reflecting zygote and embryo quality in polycystic ovary syndrome. Arch Gynecol Obstet N/A:N/A (2021). PubMed: 33772329
  • Xia S  et al. Delta-like 4 is required for pulmonary vascular arborization and alveolarization in the developing lung. JCI Insight 6:N/A (2021). PubMed: 33830085
  • Fan J  et al. Targeting the Notch and TGF-ß signaling pathways to prevent retinal fibrosis in vitro and in vivo. Theranostics 10:7956-7973 (2020). PubMed: 32724452
  • Sun W  et al. Dexmedetomidine Provides Protection Against Hippocampal Neuron Apoptosis and Cognitive Impairment in Mice with Alzheimer's Disease by Mediating the miR-129/YAP1/JAG1 Axis. Mol Neurobiol 57:5044-5055 (2020). PubMed: 32839917
  • Zhang M  et al. ZEB1-activated LINC01123 accelerates the malignancy in lung adenocarcinoma through NOTCH signaling pathway. Cell Death Dis 11:981 (2020). PubMed: 33191397
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 24 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cell (limbal stem cell)
Permeabilization
Yes - 1%BSA + 1% Triton X-100
Specification
limbal stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Aug 23 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jul 01 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 10 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Spleen
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 10 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoids)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 Organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Feb 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Jagged1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Jagged1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jan 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Jagged1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

MR. David Ivancic

Verified customer

提交于 Jul 01 2019

Western blot abreview for Anti-Jagged1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Newborn kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Newborn kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Mar 01 2018

Flow Cytometry abreview for Anti-Jagged1 antibody

Poor
Abreviews
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (Skin)
Permeabilization
No
Specification
Skin
Read More

Abcam user community

Verified customer

提交于 Nov 20 2015

1-10 of 24 Abreviews or Q&A

  •  向后
  • 1
  • 2
  • 3
  • 向前 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2022 艾博抗(上海)贸易有限公司版权所有