- 数据表
- 具体的参考文献 (30)
- 实验方案
概述
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产品名称Anti-Jagged1抗体
参阅全部 Jagged1 一抗 -
描述兔多克隆抗体to Jagged1
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宿主Rabbit
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经测试应用适用于: ICC/IF, IHC-P, WBmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab7771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/100. | |
| WB | 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate and a 75 kDa band in human brain and kidney lysates. |
靶标
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功能Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
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组织特异性Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.
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疾病相关Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
序列相似性Contains 1 DSL domain.
Contains 15 EGF-like domains. -
发展阶段Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 182 Human
- Entrez Gene: 16449 Mouse
- Entrez Gene: 29146 Rat
- Omim: 601920 Human
- SwissProt: P78504 Human
- SwissProt: Q9QXX0 Mouse
- SwissProt: Q63722 Rat
- Unigene: 224012 Human
see all -
别名
- AGS antibody
- AHD antibody
- AWS antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast LaboratoryFormalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
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Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)This image is courtesy of Aihua Ma, Univdersity of CardiffStaining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast LaboratoryFormalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin -
Western blot - Anti-Jagged1 antibody (ab7771)ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
Exposure time: 1 minute. Expected molecular weight: 134 kDaLanes 1 to 3 and 5: 20µg of cell lysate per lane
Lane 4: 20 µl of 1x LDS loading bufferLane 1: Human Brain tissue lysate
Lane 2: Human Kidney tissue Lysate
Lane 3: Human liver tissue lysate
Lane 4: 1x LDS loading buffer
Lane 5: Mouse liver lysate
实验方案
文献
This product has been referenced in:
- Luo X et al. Inhibition of notch signaling pathway temporally postpones the cartilage degradation progress of temporomandibular joint arthritis in mice. J Craniomaxillofac Surg 46:1132-1138 (2018). Read more (PubMed: 29779621) »
- Marathe S et al. Jagged1 Is Altered in Alzheimer's Disease and Regulates Spatial Memory Processing. Front Cell Neurosci 11:220 (2017). Read more (PubMed: 28848392) »
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