概述

  • 产品名称
  • 描述
    兔多克隆抗体to JAB1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Xenopus tropicalis
  • 免疫原

    Recombinant full length protein corresponding to Human JAB1 aa 1-334. (With tag; BC001187).
    Sequence:

    MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHH YFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPV EGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGI DVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGP SEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTL SSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKL AKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS


    Database link: Q92905

  • 阳性对照
    • Human colon cancer tissue.

性能

应用

Our Abpromise guarantee covers the use of ab182756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
IHC-P 1/150 - 1/500.

靶标

  • 功能
    Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.
  • 序列相似性
    Belongs to the peptidase M67A family. CSN5 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • 结构域
    The JAMM motif is essential for the protease activity of the CSN complex resulting in deneddylation of cullins. It constitutes the catalytic center of the complex.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 38 kDa Mov34 homolog antibody
    • COP9 (constitutive photomorphogenic) homolog subunit 5 antibody
    • COP9 constitutive photomorphogenic homolog subunit 5 antibody
    • COP9 signalosome complex subunit 5 antibody
    • COP9 signalosome subunit 5 antibody
    • Cop9 subunit 5 antibody
    • COPS 5 antibody
    • cops5 antibody
    • CSN 5 antibody
    • CSN5 antibody
    • CSN5_HUMAN antibody
    • JAB 1 antibody
    • Jun activation domain binding protein 1 antibody
    • Jun activation domain binding protein antibody
    • Jun activation domain-binding protein 1 antibody
    • MGC3149 antibody
    • MOV 34 antibody
    • MOV34 antibody
    • MOV34 family, 38-KD member antibody
    • SGN 5 antibody
    • SGN5 antibody
    • Signalosome subunit 5 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling JAB1 with ab182756 at 1/120 dilution (left panel). Right panel was treated with the immunogen.

  • Anti-JAB1 antibody (ab182756) at 1/550 dilution + Mouse liver tissue at 30 µg

    Secondary
    Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 5 seconds


    Gel: 12% SDS-PAGE

文献

This product has been referenced in:
  • Liu J  et al. A genome-scale CRISPR-Cas9 screening in myeloma cells identifies regulators of immunomodulatory drug sensitivity. Leukemia N/A:N/A (2018). Read more (PubMed: 30026574) »
See 1 Publication for this product

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