Isocitrate Dehydrogenase Assay试剂盒(Colorimetric) (ab102528)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 45 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
概述
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产品名称
Isocitrate Dehydrogenase Assay试剂盒(Colorimetric)
参阅全部 Isocitrate Dehydrogenase 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Enzyme activity -
灵敏度
> 0.01 mU/well -
检测时间
0h 45m -
产品概述
Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.
Visit our FAQs page for tips and troubleshooting.
Isocitrate dehydrogenase assay protocol summary:
- add samples and standards to wells
- incubate for 3 min and analyze with microplate reader
- incubate for 30 min - 2 hr and analyze again -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K756 Isocitrate Dehydrogenase Activity Colorimetric Assay Kit. K756-100 is the same size as the 100 test size of ab102528.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests β-NADP Stock 1 vial Developer Solution II 1 vial IDH Positive Control 1 x 20µl IDH Substrate Mix 1 vial Isocitrate Assay Buffer 1 x 25ml NAD+ 1 vial NADH Standard I 1 vial -
研究领域
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相关性
Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+. -
细胞定位
Cytoplasm. Peroxisome -
别名
- Cytosolic NADP isocitrate dehydrogenase
- EC=1.1.1.42
- IDH
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab102528 被引用在 17 文献中.
- Ming-Chin Lee K et al. Type I interferon antagonism of the JMJD3-IRF4 pathway modulates macrophage activation and polarization. Cell Rep 39:110719 (2022). PubMed: 35443173
- Chocron ES et al. Mitochondrial TrxR2 regulates metabolism and protects from metabolic disease through enhanced TCA and ETC function. Commun Biol 5:467 (2022). PubMed: 35577894
- Vaziri-Gohar A et al. Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors. Nat Cancer 3:852-865 (2022). PubMed: 35681100
- Doulias PT et al. TCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use. J Neurovirol 27:367-378 (2021). PubMed: 33876414
- Liu X et al. The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry. Signal Transduct Target Ther 6:375 (2021). PubMed: 34728602