Anti-IDH1抗体(ab137568)
Key features and details
- Rabbit polyclonal to IDH1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IDH1抗体
参阅全部 IDH1 一抗 -
描述
兔多克隆抗体to IDH1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human IDH1 aa 30-337.
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阳性对照
- WB: A431, HepG2, JC, or C2C12 cell lysates IHC-P: Human Colon carcinoma tissue; Mouse brain tissue; Rat duodenum tissue.
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常规说明
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137568于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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WB |
1/500 - 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).
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说明 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
WB
1/500 - 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa). |
靶标
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疾病相关
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
序列相似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻译后修饰
Acetylation at Lys-374 dramatically reduces catalytic activity. -
细胞定位
Cytoplasm. Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 3417 Human
- Entrez Gene: 15926 Mouse
- Entrez Gene: 24479 Rat
- Omim: 147700 Human
- SwissProt: O75874 Human
- SwissProt: O88844 Mouse
- SwissProt: P41562 Rat
- Unigene: 593422 Human
see all -
别名
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
图片
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All lanes : Anti-IDH1 antibody (ab137568) at 1/500 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : IDH1 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa -
All lanes : Anti-IDH1 antibody (ab137568) at 1/1000 dilution
Lane 1 : JC whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
10% SDS PAGE -
All lanes : Anti-IDH1 antibody (ab137568) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin embedded human colon carcinoma, labelling IDH1 with ab137568 at 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded mouse brain tissue labelling IDH1 with ab137568 at 1/1000 dilution. Antigen retrieval was performed with citrate buffer, pH 6.0, 15 minutes.
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Immunohistochemical analysis of paraffin embedded rat duodenum tissue labelling IDH1 with ab137568 at 1/1000 dilution. Antigen retrieval was performed with citrate buffer, pH 6.0, 15 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab137568 尚未被引用在任何文献中。