Anti-IRF5抗体(ab2932)

Reviews (4)Q&A (1)References (14)
Western blot - Anti-IRF5 antibody (ab2932)

    Key features and details

    • Goat polyclonal to IRF5
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    重组抗体的批间可重复性更强

    Product image
    Anti-IRF5 antibody [EPR17067] (ab181553)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-IRF5抗体
      参阅全部 IRF5 一抗

    • 描述

      山羊多克隆抗体to IRF5

    • 宿主

      Goat

    • 特异性

      This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1).

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Cow

    • 免疫原

      Synthetic peptide: QGPWPMHPAGMQ, corresponding to C terminal amino acids 493-504 of Human IRF5.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

    • 常规说明

      Principal Names – IRF5; interferon regulatory factor 5 Official Gene Symbol - IRF5 GenBank Accession Number – NP_002191; NP_116032 LocusLink ID - 3663 (human) Gene Ontology terms - RNA polymerase III transcription factor; cell growth and/or maintenance

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2932 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55,60,65 kDa (predicted molecular weight: 58 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 疾病相关

      Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.

    • 序列相似性

      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Interferon regulatory factor 5 antibody
      • Interferon regulatory factor 5 bone marrow variant antibody
      • IRF 5 antibody
      • IRF-5 antibody
      • Irf5 antibody
      • IRF5_HUMAN antibody
      • SLEB10 antibody
      see all

    图片

    • Western blot - Anti-IRF5 antibody (ab2932)
      Western blot - Anti-IRF5 antibody (ab2932)
      All lanes : Anti-IRF5 antibody (ab2932) at 1 µg/ml

      Lane 1 : Human Spleen (in RIPA buffer)
      Lane 2 : A549 cell lysate (in RIPA buffer)
      Lane 3 : A549 cell lysate (in RIPA buffer) with peptide

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 58 kDa
      Observed band size: 55,60,65 kDa
      why is the actual band size different from the predicted?



      Primary incubation 1 hour at room temperature. Detected by chemiluminescence.

    文献 (14)

    发表研究结果有使用 ab2932？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2932 被引用在 14 文献中.

    • Rodríguez M  et al. Fungal pattern receptors down-regulate the inflammatory response by a cross-inhibitory mechanism independent of interleukin-10 production. Immunology 150:184-198 (2017). PubMed: 27709605
    • Cushing L  et al. IRAK4 kinase activity controls Toll-like receptor-induced inflammation through the transcription factor IRF5 in primary human monocytes. J Biol Chem 292:18689-18698 (2017). PubMed: 28924041
    • Li D  et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep 6:31002 (2016). PubMed: 27481535
    • Massimino M  et al. IRF5 is a target of BCR-ABL kinase activity and reduces CML cell proliferation. Carcinogenesis 35:1132-43 (2014). Human . PubMed: 24445143
    • McEwan WA  et al. Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat Immunol 14:327-36 (2013). PubMed: 23455675
    View all Publications for this product

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-IRF5 antibody - ChIP Grade

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Dog Cell lysate - whole cell (CPEK)
    Specification
    CPEK
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 12 2014

    Western blot abreview for Anti-IRF5 antibody - ChIP Grade

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing (10 % sds)
    Sample
    Human Cell lysate - whole cell (MDM)
    Specification
    MDM
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 21 2014

    Western blot abreview for Anti-IRF5 antibody - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Mouse Cell lysate - whole cell (BMDM)
    Specification
    BMDM
    Treatment
    100 nM LPS, 24 h
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 20 2014

    Western blot abreview for Anti-IRF5 antibody - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (monocyte-derived dendritic cells)
    Loading amount
    30 µg
    Specification
    monocyte-derived dendritic cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 17 2013

    Question


    Inquiry: Hi, I need and antibody for Mouse IRF5-ChIP grade. I notice that Abcam has a human IRF5 specific (predicted to work on mouse too). Do you have updated data for this antibody to work in mice? Can you send me a sample to try it? I have access to IRF5 knockout mice that I can use as a control.

    Read More
    Answer

    Thank you very much for your interest in ab2932.

    To our knowledge, ab2932 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab2932 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab2932 in mouse. I will then send a discount code. This code must be issued before purchasing ab2932 so please wait for my reply before ordering.

    2. Purchase ab2932 either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2932 turns out to be unsuitable for mouse you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com