Anti-IRF5抗体(ab2932)
Key features and details
- Goat polyclonal to IRF5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IRF5抗体
参阅全部 IRF5 一抗 -
描述
山羊多克隆抗体to IRF5 -
宿主
Goat -
特异性
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Synthetic peptide: QGPWPMHPAGMQ, corresponding to C terminal amino acids 493-504 of Human IRF5.
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阳性对照
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常规说明
Principal Names – IRF5; interferon regulatory factor 5 Official Gene Symbol - IRF5 GenBank Accession Number – NP_002191; NP_116032 LocusLink ID - 3663 (human) Gene Ontology terms - RNA polymerase III transcription factor; cell growth and/or maintenance
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55,60,65 kDa (predicted molecular weight: 58 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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疾病相关
Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. -
序列相似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 615340 Cow
- Entrez Gene: 3663 Human
- Entrez Gene: 27056 Mouse
- Entrez Gene: 296953 Rat
- Omim: 607218 Human
- SwissProt: Q58DJ0 Cow
- SwissProt: Q13568 Human
- SwissProt: P56477 Mouse
see all -
别名
- Interferon regulatory factor 5 antibody
- Interferon regulatory factor 5 bone marrow variant antibody
- IRF 5 antibody
see all
图片
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Western blot - Anti-IRF5 antibody (ab2932)All lanes : Anti-IRF5 antibody (ab2932) at 1 µg/ml
Lane 1 : Human Spleen (in RIPA buffer)
Lane 2 : A549 cell lysate (in RIPA buffer)
Lane 3 : A549 cell lysate (in RIPA buffer) with peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
Observed band size: 55,60,65 kDa why is the actual band size different from the predicted?Primary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Western blot - Anti-IRF5 antibody (ab2932)ab2932 staining (2µ g/ml) of A549 lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
数据表及文件
文献 (14)
ab2932 被引用在 14 文献中.
- Rodríguez M et al. Fungal pattern receptors down-regulate the inflammatory response by a cross-inhibitory mechanism independent of interleukin-10 production. Immunology 150:184-198 (2017). PubMed: 27709605
- Cushing L et al. IRAK4 kinase activity controls Toll-like receptor-induced inflammation through the transcription factor IRF5 in primary human monocytes. J Biol Chem 292:18689-18698 (2017). PubMed: 28924041
- Li D et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep 6:31002 (2016). PubMed: 27481535
- Massimino M et al. IRF5 is a target of BCR-ABL kinase activity and reduces CML cell proliferation. Carcinogenesis 35:1132-43 (2014). Human . PubMed: 24445143
- McEwan WA et al. Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat Immunol 14:327-36 (2013). PubMed: 23455675
