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Immunology Innate Immunity Cytokines Interleukins

Anti-IRAK4抗体(ab5985)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (1)

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Western blot - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
  • Western blot - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

Key features and details

  • Rabbit polyclonal to IRAK4
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-IRAK4抗体
    参阅全部 IRAK4 一抗
  • 描述

    兔多克隆抗体to IRAK4
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific to IRAK4 and does not cross react with other IRAK proteins.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human IRAK4 (C terminal).
    (Peptide available as ab39782)

  • 阳性对照

    • WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IRAK4 protein (ab79739)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5985于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa.
说明
ICC/IF
Use a concentration of 10 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa.

靶标

  • 功能

    Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
  • 疾病相关

    Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
    Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.
  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q9NWZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 51135 Human
    • Omim: 606883 Human
    • SwissProt: Q9NWZ3 Human
    • Unigene: 138499 Human
    • 别名

      • IL-1 receptor-associated kinase 4 antibody
      • Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
      • Interleukin-1 receptor-associated kinase 4 antibody
      • Interleukin1 receptor associated kinase 4 antibody
      • IPD1 antibody
      • IRAK 4 antibody
      • IRAK-4 antibody
      • IRAK4 antibody
      • IRAK4 mutated form 1 antibody
      • IRAK4_HUMAN antibody
      • LOC 51135 antibody
      • NY REN 64 antibody
      • NY REN 64 antigen antibody
      • NY-REN-64 antibody
      • REN64 antibody
      • Renal carcinoma antigen NY-REN-64 antibody
      see all

    图片

    • Western blot - Anti-IRAK4 antibody (ab5985)
      Western blot - Anti-IRAK4 antibody (ab5985)
      All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

      Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    • Western blot - Anti-IRAK4 antibody (ab5985)
      Western blot - Anti-IRAK4 antibody (ab5985)
      Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
      Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
      Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml

      All lanes : HeLa cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution


      Primary incbation: 1 hour at room temperature in 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)

      Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreview
      ab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
      Left image: DAPI staining nuclei (blue)
      Middle image: IRAK4 (red)
      Right image: Overlay

      See Abreview

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab5985?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5985 被引用在 1 文献中.

    • Kallapur SG  et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . PubMed: 18056396

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-IRAK4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (PMN)
    Specification
    PMN
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 29 2009

    Western blot abreview for Anti-IRAK4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MRC-5)
    Loading amount
    1e+006 cells
    Specification
    MRC-5
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 17 2006

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