Anti-IRAK4抗体(ab5985)
Key features and details
- Rabbit polyclonal to IRAK4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-IRAK4抗体
参阅全部 IRAK4 一抗 -
描述
兔多克隆抗体to IRAK4 -
宿主
Rabbit -
特异性
This antibody is specific to IRAK4 and does not cross react with other IRAK proteins. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IRAK4 (C terminal).
(Peptide available asab39782) -
阳性对照
- WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
-
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. |
靶标
-
功能
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
疾病相关
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
-
别名
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
图片
-
Western blot - Anti-IRAK4 antibody (ab5985)All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
-
Western blot - Anti-IRAK4 antibody (ab5985)Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml
All lanes : HeLa cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incbation: 1 hour at room temperature in 5% NFDM/TBST.
-
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
-
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreviewab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)
Right image: Overlay
实验方案
数据表及文件
文献 (1)
ab5985 被引用在 1 文献中.
- Kallapur SG et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . PubMed: 18056396
