Anti-IRAK2抗体(ab6148)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to IRAK2
  • 宿主
    Rabbit
  • 特异性
    Anti-IRAK2 has no cross response to IRAK.
  • 经测试应用
    适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.

  • 阳性对照
    • Whole cell lysate from HeLa or K562 cells.
  • 常规说明


    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度
    Affinity purified
  • 纯化说明
    Purified IgG was prepared by affinity chromatography.
  • Primary antibody说明
    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.Can be blocked with IRAK2 peptide (ab6232).
IP Use at 1 µg/mg of lysate.

靶标

  • 功能
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • 组织特异性
    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • 结构域
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • 数据库链接
  • 别名
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

图片

  • Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
  • All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

  • ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF

  • Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

文献

ab6148 has not yet been referenced specifically in any publications.

客户评价及客户问答

We do not have any reports of this antibody being used for immunoprecipitation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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