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Immunology Innate Immunity Cytokines Interleukins

Anti-IRAK2抗体(ab6148)

  • Datasheet
Reviews (1)Q&A (1)

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Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
  • Western blot - Anti-IRAK2 antibody (ab6148)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)

Key features and details

  • Rabbit polyclonal to IRAK2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-IRAK2抗体
    参阅全部 IRAK2 一抗
  • 描述

    兔多克隆抗体to IRAK2
  • 宿主

    Rabbit
  • 特异性

    Anti-IRAK2 has no cross response to IRAK.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.

  • 常规说明


    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Purified IgG was prepared by affinity chromatography.
  • Primary antibody说明

    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Immunology
    • Innate Immunity
    • TLR Signaling

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant human IRAK2 protein (ab60859)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6148于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 2 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa.
说明
ICC/IF
Use a concentration of 2 µg/ml.
WB
1/500 - 1/1000. Predicted molecular weight: 65 kDa.

靶标

  • 功能

    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • 组织特异性

    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • 结构域

    The protein kinase domain is predicted to be catalytically inactive.
  • Target information above from: UniProt accession O43187 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3656 Human
    • Omim: 603304 Human
    • SwissProt: O43187 Human
    • Unigene: 449207 Human
    • 别名

      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunofluorescent analysis of Hela cells staining IRAK2 with ab6148 at 20µg/ml
    • Western blot - Anti-IRAK2 antibody (ab6148)
      Western blot - Anti-IRAK2 antibody (ab6148)
      All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : K562 whole cell lysate

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)

      ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF

    • Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab6148)
      Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab6148?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6148 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-IRAK2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    1000 µg
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 07 2013

    Question

    I was wondering had you assessed the anti IRAK-2 antibody for its ability to immunoprecipitate IRAK-2 ??

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2001

    Answer

    We do not have any reports of this antibody being used for immunoprecipitation

    Read More

    Abcam Scientific Support

    回复于 May 14 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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