- 数据表
- 具体的参考文献 (19)
- 实验方案
概述
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产品名称Anti-IP3 receptor抗体
参阅全部 IP3 receptor 一抗 -
描述兔多克隆抗体to IP3 receptor
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宿主Rabbit
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经测试应用适用于: IP, WB, ICC/IF, ICC, IHC-Fr, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Hamster, Dog, Human
预测可用于: Cow
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免疫原
Synthetic peptide corresponding to Human IP3 receptor aa 1829-1848.
Sequence:NKKKDDEVDRDAPSRKKAKE
(Peptide available asab5839)
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
应用
Our Abpromise guarantee covers the use of ab5804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 314 kDa.Can be blocked with IP3 receptor peptide (ab5839). | |
| ICC/IF | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | 1/250. Staining of IP3R-I in rat cerebellum with ab5804 results in staining of Purkinje cells. |
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| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.
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组织特异性Widely expressed.
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疾病相关Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory.
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序列相似性Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
结构域The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
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翻译后修饰Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
细胞定位Endoplasmic reticulum membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 317697 Cow
- Entrez Gene: 3708 Human
- Entrez Gene: 16438 Mouse
- Entrez Gene: 25262 Rat
- Omim: 147265 Human
- SwissProt: Q9TU34 Cow
- SwissProt: Q14643 Human
- SwissProt: P11881 Mouse
see all -
别名
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-IP3 receptor antibody (ab5804)Shows immunolocalization of ITPR1 in rat cerebellum using ab5804.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody (ab5804)IHC image of ab5804 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5804, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
文献
This product has been referenced in:
- Ausman J et al. Ceramide-induced BOK promotes mitochondrial fission in preeclampsia. Cell Death Dis 9:298 (2018). Read more (PubMed: 29463805) »
- Zhao X et al. Calcium-sensing receptor-mediated L-tryptophan-induced secretion of cholecystokinin and glucose-dependent insulinotropic peptide in swine duodenum. J Vet Sci 19:179-187 (2018). Read more (PubMed: 29284209) »
客户评价及客户问答
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