Anti-GluA4抗体(ab115322)
Key features and details
- Goat polyclonal to GluA4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GluA4抗体
参阅全部 GluA4 一抗 -
描述
山羊多克隆抗体to GluA4 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human GluA4 aa 250-350 (internal sequence). (NP_000820.3; NP_001070711.1; NP_001070712.1)
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阳性对照
- Human Brain, Cortex; Human Cerebellum lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa). |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
序列相似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily. -
翻译后修饰
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 396137 Chicken
- Entrez Gene: 529904 Cow
- Entrez Gene: 100061561 Horse
- Entrez Gene: 2893 Human
- Entrez Gene: 14802 Mouse
- Entrez Gene: 29629 Rat
- Omim: 138246 Human
- SwissProt: P48058 Human
see all -
别名
- AMPA 4 antibody
- AMPA selective glutamate receptor 4 antibody
- AMPA-selective glutamate receptor 4 antibody
see all
图片
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Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115322 at 5 µg/ml, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-GluA4 antibody (ab115322) at 0.3 µg/ml (incubated for 1 hour) + Human Cerebellum lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 101 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab115322 被引用在 3 文献中.
- Shao S et al. N-Cadherin Regulates GluA1-Mediated Depressive-Like Behavior in Adolescent Female Rat Offspring following Prenatal Stress. Neuroendocrinology 112:493-509 (2022). PubMed: 34348318
- Rogers EJ et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). PubMed: 30842726
- Polepalli JS et al. Modulation of excitation on parvalbumin interneurons by neuroligin-3 regulates the hippocampal network. Nat Neurosci 20:219-229 (2017). PubMed: 28067903