Anti-Involucrin抗体[SY8] (ab20202)
Key features and details
- Mouse monoclonal [SY8] to Involucrin
- Suitable for: IP, IHC-P, WB
- Reacts with: Human, Pig, Gorilla
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Involucrin抗体[SY8]
参阅全部 Involucrin 一抗 -
描述
小鼠单克隆抗体[SY8] to Involucrin -
宿主
Mouse -
特异性
Keratinocytes of epidermis and other stratified squamous epithelia -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human, Pig, Gorilla -
免疫原
Pure involucrin from human keratinocytes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
SY8 -
骨髓瘤
P3x63 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab20202于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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说明 |
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IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. -
组织特异性
Keratinocytes of epidermis and other stratified squamous epithelia. -
序列相似性
Belongs to the involucrin family. -
翻译后修饰
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide. -
细胞定位
Cytoplasm. Constituent of the scaffolding of the cornified envelope. - Information by UniProt
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数据库链接
- Entrez Gene: 3713 Human
- Entrez Gene: 407242 Pig
- Omim: 147360 Human
- SwissProt: P07476 Human
- SwissProt: P18175 Pig
- Unigene: 516439 Human
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别名
- INVO_HUMAN antibody
- Involucrin antibody
- IVL antibody
图片
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Ab20202 staining Human skin Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab20202 被引用在 2 文献中.
- Goren I et al. Increase of cystathionine-?-lyase (CSE) during late wound repair: Hydrogen sulfide triggers cytokeratin 10 expression in keratinocytes. Nitric Oxide 87:31-42 (2019). PubMed: 30862476
- Buerger C et al. Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation. PLoS One 12:e0180853 (2017). Human . PubMed: 28700632