Anti-Interferon beta抗体(ab85803)
Key features and details
- Rabbit polyclonal to Interferon beta
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Interferon beta抗体
参阅全部 Interferon beta 一抗 -
描述
兔多克隆抗体to Interferon beta -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Macaque monkey -
免疫原
Synthetic peptide corresponding to Human Interferon beta aa 1-100 conjugated to keyhole limpet haemocyanin.
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阳性对照
- Recombinant human Interferon beta protein (ab71475) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85803于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Has antiviral, antibacterial and anticancer activities. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3456 Human
- Entrez Gene: 15977 Mouse
- Omim: 147640 Human
- SwissProt: P01574 Human
- SwissProt: P01575 Mouse
- Unigene: 93177 Human
- Unigene: 1245 Mouse
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别名
- beta-interferon antibody
- Fibroblast interferon antibody
- IFB antibody
see all
图片
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All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :WI-38 whole cell lysate (ab3960)
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : Lung (Human) Tissue Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lane 6 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted. -
ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab85803 被引用在 5 文献中.
- Abdelmoaty M et al. Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions. bioRxiv N/A:N/A (2021). PubMed: 34268510
- Abdelmoaty MM et al. Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions. Front Immunol 12:741502 (2021). PubMed: 34671355
- Li W et al. Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone. Theranostics 10:9032-9049 (2020). PubMed: 32802177
- Choi MR et al. Chloroquine Treatment Suppresses Mucosal Inflammation in a Mouse Model of Eosinophilic Chronic Rhinosinusitis. Allergy Asthma Immunol Res 12:994-1011 (2020). PubMed: 32935491
- Oishi I et al. Efficient production of human interferon beta in the white of eggs from ovalbumin gene-targeted hens. Sci Rep 8:10203 (2018). PubMed: 29976933