Anti-Interferon alpha 2b抗体[9D3] (ab9386)
Key features and details
- Mouse monoclonal [9D3] to Interferon alpha 2b
- Suitable for: Sandwich ELISA, Neutralising
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Interferon alpha 2b抗体[9D3]
参阅全部 Interferon alpha 2b 一抗 -
描述
小鼠单克隆抗体[9D3] to Interferon alpha 2b -
宿主
Mouse -
经测试应用
适用于: Sandwich ELISA, Neutralisingmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Interferon alpha 2b.
Database link: P01563 -
阳性对照
- WB: Human interferon-alpha 2b.
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常规说明
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR314306-1 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
9D3 -
骨髓瘤
NS0/1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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Sandwich ELISA |
Use at an assay dependent concentration.
This antibody can be used in a sandwich ELISA for the determination of human IFN-a2b. It recognizes different epitopes than anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258). We recommend using this antibody as a capture antibody and ab5258 as a detection antibody. |
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Neutralising |
Use at an assay dependent concentration.
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说明 |
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Sandwich ELISA
Use at an assay dependent concentration. This antibody can be used in a sandwich ELISA for the determination of human IFN-a2b. It recognizes different epitopes than anti-Interferon alpha 2b antibody [4E10] (HRP) (ab5258). We recommend using this antibody as a capture antibody and ab5258 as a detection antibody. |
Neutralising
Use at an assay dependent concentration. |
靶标
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功能
Produced by macrophages, IFN-alpha have antiviral activities. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
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别名
- Alpha 2a interferon antibody
- IFN alpha 2b antibody
- IFN-alpha-2 antibody
see all
图片
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Standard curve for the determination of human interferon-alpha 2b by two-epitope ELISA: capture antibody 9D3 (ab9386), peroxidase-labeled antibody 4E10 (ab9388).
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Neutralizing activity of monoclonal antibody 9D3 in vitro. Interferon antiviral bioassay: L41 cells, mouse encephalomyacarditis virus (EMCV). Antibodies at 5 µg/ml. Control 1 - without EMCV (non-infected cells), control 2 - without interferon, control 3 - irrelevant antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab9386 被引用在 10 文献中.
- Qin X et al. Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b. J Pharm Anal 12:308-316 (2022). PubMed: 35582401
- Najdanovic JG et al. Vascularization and osteogenesis in ectopically implanted bone tissue-engineered constructs with endothelial and osteogenic differentiated adipose-derived stem cells. World J Stem Cells 13:91-114 (2021). PubMed: 33584982
- Landowski CP et al. Enabling low cost biopharmaceuticals: high level interferon alpha-2b production in Trichoderma reesei. Microb Cell Fact 15:104 (2016). PubMed: 27287473
- Chen J et al. Dissolving microneedle-based intradermal delivery of interferon-a-2b. Drug Dev Ind Pharm 42:890-6 (2016). PubMed: 26467418
- Podobnik B et al. Conjugation of PolyPEG to interferon alpha extends serum half-life while maintaining low viscosity of the conjugate. Bioconjug Chem 26:452-9 (2015). PubMed: 25629733
- Landowski CP et al. Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei. PLoS One 10:e0134723 (2015). PubMed: 26309247
- Zhang Q et al. Expression of hepatitis B virus surface antigen determinants in Lactococcus lactis for oral vaccination. Microbiol Res 166:111-20 (2011). WB . PubMed: 20227266
- Zhang Q et al. Improvement of human interferon alpha secretion by Lactococcus lactis. Biotechnol Lett 32:1271-7 (2010). WB . PubMed: 20431909
- Kisand K et al. Interferon autoantibodies associated with AIRE-deficiency decrease the expression of IFN-stimulated genes. Blood : (2008). PubMed: 18606876
- Arlen PA et al. Field production and functional evaluation of chloroplast-derived interferon-alpha2b. Plant Biotechnol J 5:511-25 (2007). WB ; Human . PubMed: 17490449