Anti-Integrin beta 4抗体[M126] (ab29042)
Key features and details
- Mouse monoclonal [M126] to Integrin beta 4
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Integrin beta 4抗体[M126]
参阅全部 Integrin beta 4 一抗 -
描述
小鼠单克隆抗体[M126] to Integrin beta 4 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.
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阳性对照
- Flow Cyt (intra):A431 cell lysate. WB: 804G cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
protein A chromatography. -
克隆
单克隆 -
克隆编号
M126 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab29042于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
疾病相关
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
结构域
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
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别名
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
图片
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Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)
Lane 1 : A431 (25 µg/lane) serum starved overnight
Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min
Predicted band size: 202 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab29042 被引用在 25 文献中.
- Mezawa M et al. TNF-α regulates the composition of the basal lamina and cell-matrix adhesions in gingival epithelial cells. Cell Adh Migr 16:13-24 (2022). PubMed: 35137648
- Tan ML et al. Integrin-β4 regulates the dynamic changes of phenotypic characteristics in association with epithelial-mesenchymal transition (EMT) and RhoA activity in airway epithelial cells during injury and repair. Int J Biol Sci 18:1254-1270 (2022). PubMed: 35173551
- Romo-Valera C et al. Characterisation of corneas following different time and storage methods for their use as a source of stem-like limbal epithelial cells. Exp Eye Res 211:108720 (2021). PubMed: 34389315
- Pothuraju R et al. Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance. Mol Cancer 19:37 (2020). PubMed: 32098629
- Yin H et al. Identification of Sca-1+Abcg1+ bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells. Oncogene 39:3754-3773 (2020). PubMed: 32157214