重组Anti-Integrin beta 4抗体[EPR17517] (ab182120)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17517] to Integrin beta 4
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Integrin beta 4抗体[EPR17517]
参阅全部 Integrin beta 4 一抗 -
描述
兔单克隆抗体[EPR17517] to Integrin beta 4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse spleen, kidney, pancreas, ovary and skin lysates; Human skin and pancreas lysates; BxPC-3 and SW480 whole cell lysates. IHC-P: Human colon, Human cervical carcinoma and mouse pancreas tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17517 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182120于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 210 kDa (predicted molecular weight: 202 kDa).
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说明 |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 210 kDa (predicted molecular weight: 202 kDa). |
靶标
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功能
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
组织特异性
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
疾病相关
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
序列相似性
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
结构域
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3691 Human
- Entrez Gene: 192897 Mouse
- Omim: 147557 Human
- SwissProt: P16144 Human
- SwissProt: A2A863 Mouse
- Unigene: 632226 Human
- Unigene: 213873 Mouse
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别名
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
图片
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Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: ITGB4 knockout HAP1 whole cell lysate (40 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab182120 observed at 240 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab182120 was shown to specifically recognize ITGB4 in wild-type HAP1 cells alond with additional cross-reactive bands. No band was observed when ITGB4 knockout samples were used. Wild-type and ITGB4 knockout samples were subjected to SDS-PAGE. Ab182120 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell junction staining on mouse pancreatic acinus is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human colon epithelium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution
Lane 1 : Mouse pancreas lysate
Lane 2 : Mouse ovary lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Mouse skin lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Human skin lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + Human pancreas lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/5000 dilution + SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 202 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (22)
ab182120 被引用在 22 文献中.
- Chen CY et al. KCNF1 promotes lung cancer by modulating ITGB4 expression. Cancer Gene Ther 30:414-423 (2023). PubMed: 36385523
- Li L et al. The adhesive heterogeneity of different compartments of oral mucosal rete ridges. Exp Dermatol 31:413-419 (2022). PubMed: 34665894
- Chi Y et al. Deficiency of Integrin β4 Results in Increased Lung Tissue Stiffness and Responds to Substrate Stiffness via Modulating RhoA Activity. Front Cell Dev Biol 10:845440 (2022). PubMed: 35309934
- Choi SH et al. KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer. Mol Cancer Res 20:1305-1319 (2022). PubMed: 35394541
- Zhu X et al. TCN1 Deficiency Inhibits the Malignancy of Colorectal Cancer Cells by Regulating the ITGB4 Pathway. Gut Liver N/A:N/A (2022). PubMed: 35686504