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Cardiovascular Angiogenesis Adhesion / ECM Integrins

Anti-Integrin alpha V beta 3抗体[BV3] (ab7166)

  • Datasheet
  • SDS
Reviews (10)Q&A (8)References (22)

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Key features and details

  • Mouse monoclonal [BV3] to Integrin alpha V beta 3
  • Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cyt
  • Reacts with: Rat, Chicken, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Integrin alpha V beta 3抗体[BV3]
    参阅全部 Integrin alpha V beta 3 一抗
  • 描述

    小鼠单克隆抗体[BV3] to Integrin alpha V beta 3
  • 宿主

    Mouse
  • 特异性

    This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cytmore details
    不适用于: WB
  • 种属反应性

    与反应: Rat, Chicken, Human
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    BV3
  • 同种型

    IgG1
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Beta
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Thrombocytic Lineage

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7166于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (2)
Use at an assay dependent concentration.
IHC-P (5)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

应用说明
Is unsuitable for WB.

靶标

  • 相关性

    Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
  • 细胞定位

    Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
  • 数据库链接

    • Entrez Gene: 3685 Human
    • Entrez Gene: 3690 Human
    • Entrez Gene: 29302 Rat
    • Entrez Gene: 296456 Rat
    • Omim: 173470 Human
    • Omim: 193210 Human
    • SwissProt: P05106 Human
    • SwissProt: P06756 Human
    • Unigene: 436873 Human
    see all
  • 别名

    • Antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD 61 antibody
    • CD51 antibody
    • CD51 antigen antibody
    • CD61 antibody
    • CD61 antigen antibody
    • DKFZp686A08142 antibody
    • GP3A antibody
    • GPIIIa antibody
    • Integrin alpha V antibody
    • Integrin alpha V vitronectin receptor alpha polypeptide antibody
    • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • Integrin beta 3 antibody
    • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
    • Integrin beta chain beta 3 antibody
    • integrinVbeta3 antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • MSK 8 antibody
    • MSK8 antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • Vitronectin receptor alpha polypeptide antibody
    • vitronectin receptor antibody
    • Vitronectin receptor subunit alpha antibody
    • VNR 1 antibody
    • VNR1 antibody
    • VNRA antibody
    see all

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (22)

发表研究结果有使用 ab7166?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7166 被引用在 22 文献中.

  • Lobeek D  et al. Imaging angiogenesis in patients with head and neck squamous cell carcinomas by [68Ga]Ga-DOTA-E-[c(RGDfK)]2 PET/CT. Eur J Nucl Med Mol Imaging 47:2647-2655 (2020). PubMed: 32198613
  • Zhao L  et al. Targeted Radionuclide Therapy in Patient-Derived Xenografts Using 177Lu-EB-RGD. Mol Cancer Ther 19:2034-2043 (2020). PubMed: 32847972
  • Allerkamp H  et al. Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1. Reprod Biol 19:412-420 (2019). PubMed: 31806575
  • Faralli JA  et al. Effect of avß3 Integrin Expression and Activity on Intraocular Pressure. Invest Ophthalmol Vis Sci 60:1776-1788 (2019). PubMed: 31022732
  • He F  et al. Reversal of EGFR inhibitors' resistance by co-delivering EGFR and integrin avß3 inhibitors with nanoparticles in non-small cell lung cancer. Biosci Rep 39:N/A (2019). PubMed: 31316001
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 18 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V beta 3 antibody [BV3]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate (10mM, pH 6.0)
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Ms. Noureen Ali

Verified customer

提交于 Mar 26 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V beta 3 antibody [BV3]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Cell Conditioning 1&2 (Roche Diagnostics)
Permeabilization
No
Specification
Uterus
Blocking step
Diluant Anticorps (Roche Diagnostics) as blocking agent for 32 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde
Read More

Florian Chocteau

Verified customer

提交于 Nov 29 2017

Western blot abreview for Anti-CD51 + CD61 antibody [BV3]

Inconclusive
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell line stably expressing Integrin)
Loading amount
60 µg
Specification
breast cancer cell line stably expressing Integrin
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Jan 30 2012

Question

Inquiry: I'm wondering wether this Ab recognise the intact alfaV beta3 Complex or subunit seperately. I need a Ab only reactive to entire Complex insteat of each subunit.

Read More

Abcam community

Verified customer

Asked on Sep 04 2012

Answer

Thank you for contacting us.

Unfortunately, we have not mapped the epitope of ab7166 so we haveno informationif ab7166 is against the entire complex or not.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Sep 04 2012

Question

Thank you for your reply, that is very interesting. We had selected to use CD51/61 as a marker for angiogenesis, we are looking into the pathological outcome of possible angiogenesis in brain tissue. We had selected the glioblastoma case as a positive control to test the antibody before running it through our cohort. Do you have any recommendations of how we can increase specific staining of the antibody? Do you believe then that staining of blood is just monocytes, we had suspected that it maybe red blood cells.
I look forward to hearing your response
Kind regards

Read More

Abcam community

Verified customer

Asked on Apr 03 2012

Answer

Thank you for your email.

The sensitivity of staining could be increased by;

- using enhancers; we have DAB enhancer in catalogue, please check the datasheet https://www.abcam.com/DAB-Enhancer-ab675.html
- Using lower dilution of primary or secondary abs without compromising the background
- Incubating primary overnight at 4C and secondary for 2 hours RT
- Ab Optimization; In IHC-P optimization of antibodies is always necessary for finding the right dilution and best antigen retrieval procedure e.g. sometime 5 min microwave give clean staining than 10 min microwave. In my own experience microwave 10 minutes gave cleaner staining than 5 minutes so it is good to check which experimental condition is best suitable in relation to tissue section and antibodies.

RBCs do not express CD51 and CD61 so the antibody should not bind to these cells. To confirm this you may consider dipping the slides in hematoxylin solution, RBCs do not have nucleus so they will not be stained.

The following website is a also a good resource for searching the endothelial targets; http://www.antibodybeyond.com/reviews/tumor-markers/angiogenesis-marker.htm

Let me know what you think.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 Apr 03 2012

Question

I have converted images to JPEG hopefully that will help. Please find attached images of glioblastoma case 2 images of microwave retrieval and 2 with pressure cooker, both using EDTA. Tried citrate buffer before and found similar problems. I have checked with the technicians and the case has low post mortem delay and fixation time

Kind regards

Read More

Abcam community

Verified customer

Asked on Apr 02 2012

Answer

Thank you for your email.

The CD51 and CD61 target is expressed by endothelial cells, megakaryocytes, monocytes, macrophages and Neutophils which is why the staining is more in the blood vessels.

It seems the antibody is binding to the cells where it suppose to bind however the staining is quite faint.

I suppose you are studying Glioblastoma in human brain which involves glial cells. Could I ask the reason of choosing CD51 and CD61 targets.

I will look forward to hearing from you soon.

Read More

Abcam Scientific Support

回复于 Apr 02 2012

Question

Antibody giving high background
- Looks like staining not specific to endothelial cells
- Interested in observing Gleoblastoma
- Human brain tissue sections (post mortem)
- Citrate buffer, EDTA buffer usedwith 10 minutes heating in microwave and pressure cooker.
- Ab dilution used 1/100, 1/500 and 1/1000 no improvement

Read More

Abcam community

Verified customer

Asked on Mar 30 2012

Answer

Thank you for contacting us.

As discussed on phone please attach images and send it to us.

We will look forward to hearing from you soon.

Read More

Abcam Scientific Support

回复于 Mar 30 2012

Question

I have used both of these antibodies for flow cytometry and immunohistochemistry. I saw that both can be used for immunoprecipitation. I would like to know if you all have an idea whether these antibodies may also work for western blots or you've tried them for WBs and they didn't work?

Read More

Abcam community

Verified customer

Asked on Jan 13 2012

Answer

Thank you for contacting Abcam. I have checked our notes and it appears that ab7166 is unsuitable for western blot, while ab24694 will most probably work for western blotting, its just that we have not tested it in that application. Please let me know if there is anything else I can help you with.

Read More

Abcam Scientific Support

回复于 Jan 13 2012

Question

Dear Mr. Hryca,  we work with an Antibody of you: ab7166 CD51/CD61 Clone BV3. Storage temperature is descriped 4°C. I would feel saver to make aliquots and store it at -80°C; can I do that? Thank you for your help. Best regards  

Read More

Abcam community

Verified customer

Asked on Oct 24 2011

Answer

Thank you for your enquiry. I would advise you to follow the storage instructions provided on the datahseet. Each product has specific instruction for shipping and storage. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. If you need any further assistance in the future, please do not hesitate to contact me.        

Read More

Abcam Scientific Support

回复于 Oct 24 2011

Question

I am looking for an antibody to any cell surface marker that will work on non-permeabilized HeLa cells. Any suggestions?  

Read More

Abcam community

Verified customer

Asked on Apr 20 2011

Answer

Thank you for contacting us. I have looked at a number of candidates for cell surface markers for HeLa cells, but I am not aware of any that are unique to HeLa cells, if you are trying to separate them from a mixed population. The following paper discusses CD51 expression on HeLa cells: Quantitation and kinetics of CD51 surface receptor expression: implications for targeted delivery. Ann Biomed Eng. 2004 May;32(5):635-44. PMID: 15171619 http://www.ncbi.nlm.nih.gov/pubmed/15171619 Our antibody ab7166 has been used for flow cytometry on non-permeabilized cells: Click here (or use the following: https://www.abcam.com/Integrin-alpha-V-beta-3-antibody-BV3-ab7166.html). Antibodies that recognize the extracellular portion of beta 1 integrin (CD29) will also label intact HeLa cells, for example ab30388: Click here (or use the following: https://www.abcam.com/Integrin-beta-1-antibody-JB1B-ab30388.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Apr 20 2011

1-10 of 18 Abreviews or Q&A

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