Anti-Integrin alpha V beta 3抗体[BV3] (ab7166)

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Unfortunately, we have not mapped the epitope of ab7166 so we haveno informationif ab7166 is against the entire complex or not.


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The sensitivity of staining could be increased by;

- using enhancers; we have DAB enhancer in catalogue, please check the datasheet https://www.abcam.com/DAB-Enhancer-ab675.html
- Using lower dilution of primary or secondary abs without compromising the background
- Incubating primary overnight at 4C and secondary for 2 hours RT
- Ab Optimization; In IHC-P optimization of antibodies is always necessary for finding the right dilution and best antigen retrieval procedure e.g. sometime 5 min microwave give clean staining than 10 min microwave. In my own experience microwave 10 minutes gave cleaner staining than 5 minutes so it is good to check which experimental condition is best suitable in relation to tissue section and antibodies.

RBCs do not express CD51 and CD61 so the antibody should not bind to these cells. To confirm this you may consider dipping the slides in hematoxylin solution, RBCs do not have nucleus so they will not be stained.

The following website is a also a good resource for searching the endothelial targets; http://www.antibodybeyond.com/reviews/tumor-markers/angiogenesis-marker.htm

Let me know what you think.

I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

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The CD51 and CD61 target is expressed by endothelial cells, megakaryocytes, monocytes, macrophages and Neutophils which is why the staining is more in the blood vessels.

It seems the antibody is binding to the cells where it suppose to bind however the staining is quite faint.

I suppose you are studying Glioblastoma in human brain which involves glial cells. Could I ask the reason of choosing CD51 and CD61 targets.

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As discussed on phone please attach images and send it to us.

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Thank you for contacting Abcam. I have checked our notes and it appears that ab7166 is unsuitable for western blot, while ab24694 will most probably work for western blotting, its just that we have not tested it in that application. Please let me know if there is anything else I can help you with.

Thank you for your enquiry. I would advise you to follow the storage instructions provided on the datahseet. Each product has specific instruction for shipping and storage. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. If you need any further assistance in the future, please do not hesitate to contact me.        

Thank you for contacting us. I have looked at a number of candidates for cell surface markers for HeLa cells, but I am not aware of any that are unique to HeLa cells, if you are trying to separate them from a mixed population. The following paper discusses CD51 expression on HeLa cells: Quantitation and kinetics of CD51 surface receptor expression: implications for targeted delivery. Ann Biomed Eng. 2004 May;32(5):635-44. PMID: 15171619 http://www.ncbi.nlm.nih.gov/pubmed/15171619 Our antibody ab7166 has been used for flow cytometry on non-permeabilized cells: Click here (or use the following: https://www.abcam.com/Integrin-alpha-V-beta-3-antibody-BV3-ab7166.html). Antibodies that recognize the extracellular portion of beta 1 integrin (CD29) will also label intact HeLa cells, for example ab30388: Click here (or use the following: https://www.abcam.com/Integrin-beta-1-antibody-JB1B-ab30388.html). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.