Key features and details
- Mouse monoclonal [44H6] to Integrin alpha 4/CD49D (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
产品名称Anti-Integrin alpha 4/CD49D抗体[44H6] (FITC)
参阅全部 Integrin alpha 4/CD49D 一抗
描述小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D (FITC)
偶联物FITC. Ex: 493nm, Em: 528nm
特异性44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation.
经测试应用适用于: Flow Cytmore details
HOON pre-B leukaemia cell line
Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.
This product was previously labelled as Integrin alpha 4
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Our Abpromise guarantee covers the use of ab221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
or 100µl whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
序列相似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
结构域The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
翻译后修饰Phosphorylation on Ser-1027 inhibits PXN binding.
- Information by UniProt
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
ab221 被引用在 2 文献中.
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Chen X et al. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res 6:34 (2018). PubMed: 30510839