Key features and details
- Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
产品名称Anti-Integrin alpha 4/CD49D抗体[44H6]
参阅全部 Integrin alpha 4/CD49D 一抗
描述小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D
Recognises the Integrin alpha 4/CD49D subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
经测试应用适用于: Flow Cyt, ICC/IFmore details
HOON pre-B leukaemia cell line.
- Flow Cyt: Human whole peripheral blood cells. IHC-Fr: Human tonsil tissue. ICC/IF: Wild-type HAP-1 cells.
This product was previously labelled as Integrin alpha 4
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.1% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明Purified IgG prepared by ion exchange chromotography from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 5 µg/ml.|
功能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
序列相似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
结构域The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
翻译后修饰Phosphorylation on Ser-1027 inhibits PXN binding.
- Information by UniProt
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
ab220 staining Integrin alpha 4/CD49D in wild-type HAP1 cells (top panel) and Integrin alpha 4/CD49D knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab220 at 5μg/ml concentration and ab220272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Staining of human peripheral blood lymphocytes with ab220.
ab220 被引用在 1 文献中.
- Jouhilahti EM et al. The development of cutaneous neurofibromas. Am J Pathol 178:500-5 (2011). PubMed: 21281783