Anti-Integrin alpha 4/CD49D抗体[44H6] (ab220)
Key features and details
- Mouse monoclonal [44H6] to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Integrin alpha 4/CD49D抗体[44H6]
参阅全部 Integrin alpha 4/CD49D 一抗 -
描述
小鼠单克隆抗体[44H6] to Integrin alpha 4/CD49D -
宿主
Mouse -
特异性
Recognises the Integrin alpha 4/CD49D subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
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Tested Applications & Species
Application Species Flow Cyt HumanICC/IF Human -
免疫原
HOON pre-B leukaemia cell line.
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阳性对照
- Flow Cyt: Human whole peripheral blood cells. IHC-Fr: Human tonsil tissue. ICC/IF: Wild-type HAP-1 cells.
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常规说明
This product was previously labelled as Integrin alpha 4
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified IgG prepared by ion exchange chromotography from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
44H6 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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ICC/IF |
Human
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应用 | Ab评论 | 说明 |
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Flow Cyt |
1/40.
Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF |
Use a concentration of 5 µg/ml.
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说明 |
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Flow Cyt
1/40. Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
结构域
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
翻译后修饰
Phosphorylation on Ser-1027 inhibits PXN binding. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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别名
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
图片
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ab220 staining Integrin alpha 4/CD49D in wild-type HAP1 cells (top panel) and Integrin alpha 4/CD49D knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab220 at 5μg/ml concentration and ab220272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
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Staining of human peripheral blood lymphocytes with ab220.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab220 被引用在 1 文献中.
- Jouhilahti EM et al. The development of cutaneous neurofibromas. Am J Pathol 178:500-5 (2011). PubMed: 21281783