使用敲除细胞株进行验证

Anti-Integrin alpha 4抗体[44H6] (ab220)

Abreviews (1)Q&A (5)Specific References (1)

概述

  • 产品名称
    Anti-Integrin alpha 4抗体[44H6]
    参阅全部 Integrin alpha 4 一抗
  • 描述
    小鼠单克隆抗体[44H6] to Integrin alpha 4
  • 宿主
    Mouse
  • 特异性
    Recognises the integrin alpha 4 subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, WB, ICC/IF, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    HOON pre-B leukaemia cell line.

  • 阳性对照
    • Flow Cyt: Human whole peripheral blood cells. IHC-Fr: Human tonsil tissue. ICC/IF: Wild-type HAP-1 cells.

性能

应用

Our Abpromise guarantee covers the use of ab220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/40.

Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr 1/50 - 1/100.
WB 1/200.
ICC/IF Use a concentration of 5 µg/ml.
IP 1/20 - 1/25.
  • 应用说明
    Is unsuitable for IHC-P.

    • 靶标

    • 功能
      Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
    • 序列相似性
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • 结构域
      The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
    • 翻译后修饰
      Phosphorylation on Ser-1027 inhibits PXN binding.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
      • 别名
        • 269C wild type antibody
        • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
        • CD49 antigen like family member D antibody
        • CD49 antigen-like family member D antibody
        • CD49d antibody
        • IA4 antibody
        • Integrin alpha 4 antibody
        • Integrin alpha 4 subunit antibody
        • Integrin alpha IV antibody
        • Integrin alpha-4 antibody
        • Integrin alpha-IV antibody
        • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
        • ITA4_HUMAN antibody
        • ITGA4 antibody
        • MGC90518 antibody
        • OTTHUMP00000205320 antibody
        • Very late activation protein 4 receptor, alpha 4 subunit antibody
        • VLA 4 subunit alpha antibody
        • VLA-4 subunit alpha antibody
        • VLA4 antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4 antibody [44H6] (ab220)
        Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 4 antibody [44H6] (ab220)

        ab220 staining Integrin alpha 4 in wild-type HAP1 cells (top panel) and Integrin alpha 4 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab220 at 5μg/ml concentration and ab220272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

      • Flow Cytometry - Anti-Integrin alpha 4 antibody [44H6] (ab220)
        Flow Cytometry - Anti-Integrin alpha 4 antibody [44H6] (ab220)
        Staining of human peripheral blood lymphocytes with ab220.

      文献

      This product has been referenced in:

      See 1 Publication for this product

      发表研究结果有使用 ab220？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

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      6 reviews or Q&A

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      Western blot abreview for Anti-Integrin alpha 4 antibody [44H6]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - other (blood Natural Killer cells)
      Loading amount
      10 µg
      Specification
      blood Natural Killer cells
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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      Abcam user community

      Verified customer

      提交于 Dec 17 2008

      Which portion of the integrin alpha4 that the antibody will detect? Is it the extracellular domain, or cytplasmic domain?

      Thank you for your enquiry. This product recognises the extracellular portion of integrin alpha 4. However, since epitope mapping was never done, there is no information on the exact epitope sequence.

      I'd like to know if this antibody recognizes the N-term region of alpha 4 integrin or which part of the protein. Thanks in advance. Best regards

      Thank you for your enquiry regarding ab220. We have not mapped the epitope region recognized by this antibody therefore we do not know which region is recognized by the antibody. Sorry I couldn't help you more on this occasion,

      follow up to previous enquiry: The two different positive controls were histag versions of human integrin alpha 4, both were loaded at approximately 50ng. It is possible there isn't enough protein in my samples but that doesn't explain why I'm not de...

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      Thank you for your email. We have had feedback from a customer who used this antibody at a 1:200 dilution for Western blotting. I'm really not sure why this antibody is not working for you, and can offer (if you have purchased the antibody in the past ...

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      follow up to previous enquiry: To answer your questions reqarding inquirey 735273 1. My transfer was good because my molecular weight markers transfer fine and I ran a number of gels at the same time and they all worked fine (different antibodys...

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      Thank you for your email. I noticed in your previous email that you mentioned for a positive control, "tried 2 different previously verified positive controls." Can you please elaborate on that? For your other samples, the soluble integrin alpha 4 secr...

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      DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Soluble integrin alpha 4 secreted into cell supernatant, lacking c-terminus which is very small PRIMARY ANTIBODY AB220 mouse anti-human 60ul/15mls BSA==4ug/ml from a 1mg/ml stock I...

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      Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Did you determine how much protein was loaded onto the gel? Also, do you know if the protein transferred properly to the membrane? What was the...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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