
Anti-Integrin alpha 4抗体[44H6] (ab220)
概述
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产品名称Anti-Integrin alpha 4抗体[44H6]
参阅全部 Integrin alpha 4 一抗 -
描述小鼠单克隆抗体[44H6] to Integrin alpha 4
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宿主Mouse
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特异性Recognises the integrin alpha 4 subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
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经测试应用适用于: Flow Cyt, IHC-Fr, ICC/IF, IPmore details
不适用于: IHC-P -
种属反应性与反应: Human
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免疫原
HOON pre-B leukaemia cell line.
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阳性对照
- Flow Cyt: Human whole peripheral blood cells. IHC-Fr: Human tonsil tissue. ICC/IF: Wild-type HAP-1 cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度Protein A purified
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纯化说明Purified IgG prepared by ion exchange chromotography from tissue culture supernatant.
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克隆单克隆
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克隆编号44H6
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骨髓瘤Sp2/0-Ag14
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同种型IgG1
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
Our Abpromise guarantee covers the use of ab220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Flow Cyt | 1/40. Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IHC-Fr | 1/50 - 1/100. | |
ICC/IF | Use a concentration of 5 µg/ml. | |
IP | 1/20 - 1/25. |
靶标
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功能Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
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序列相似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
结构域The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
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翻译后修饰Phosphorylation on Ser-1027 inhibits PXN binding.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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别名
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
图片
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ab220 staining Integrin alpha 4 in wild-type HAP1 cells (top panel) and Integrin alpha 4 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab220 at 5μg/ml concentration and ab220272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
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Staining of human peripheral blood lymphocytes with ab220.
实验方案
文献
This product has been referenced in:
- Jouhilahti EM et al. The development of cutaneous neurofibromas. Am J Pathol 178:500-5 (2011). Read more (PubMed: 21281783) »