Anti-Integrin alpha 2+beta 1抗体[P1E6] (ab24697)
Key features and details
- Mouse monoclonal [P1E6] to Integrin alpha 2+beta 1
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Integrin alpha 2+beta 1抗体[P1E6]
参阅全部 Integrin alpha 2+beta 1 一抗 -
描述
小鼠单克隆抗体[P1E6] to Integrin alpha 2+beta 1 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Integrin alpha 2+beta 1. HT1080 fibrosarcoma cells.
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阳性对照
- ICC/IF: HepG2 cells. Flow Cyt (Intra): HT1080 cells.
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常规说明
In imunofluorescence, ab24697 can be used to detect a2 in collagen dependent focal adhesions.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
ab24697 is effective in blocking adhesion cells to collagen coated surfaces. It immunoprecipitates the a2 subunit (reducing) or a2 complexed with ß1 from surface labeled cell lysates (non-reducing). In imunofluorescence, this antibody can be used to detect a2 in collagen dependent focal adhesions. -
克隆
单克隆 -
克隆编号
P1E6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
(Also PMID - 19780039) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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说明 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. (Also PMID - 19780039) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 5 - 10 µg/ml. |
靶标
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细胞定位
Cell Membrane; single-pass type I membrane protein -
数据库链接
- Entrez Gene: 3673 Human
- Entrez Gene: 3688 Human
- Omim: 135630 Human
- Omim: 192974 Human
- SwissProt: P05556 Human
- SwissProt: P17301 Human
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别名
- CD29 antibody
- CD49B antibody
- Collagen receptor antibody
see all
图片
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ab24697 staining Intergri alpha 2 + beta 1 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24697 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Overlay histogram showing HT1080 cells stained with ab24697 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HT1080 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab24697 被引用在 19 文献中.
- Potter JA et al. Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin a2ß1 Binding in TGFß1-Evoked Renal Tubular Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33807408
- Peng Y et al. Decellularized Disc Hydrogels for hBMSCs tissue-specific differentiation and tissue regeneration. Bioact Mater 6:3541-3556 (2021). PubMed: 33842740
- Pijning AE et al. An alternate covalent form of platelet αIIbβ3 integrin that resides in focal adhesions and has altered function. Blood 138:1359-1372 (2021). PubMed: 34375384
- Yu W et al. Different effects of total flavonoids from Arachniodes exilis on human umbilical cord mesenchymal stem cells in vitro. Medicine (Baltimore) 99:e20628 (2020). PubMed: 32569193
- Yen CH et al. Increased Cell Detachment Ratio of Mesenchymal-Type Lung Cancer Cells on pH-Responsive Chitosan through the ß3 Integrin. Mar Drugs 17:N/A (2019). PubMed: 31771240