为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Kits/ Lysates/ Other Tools and Reagents Western Blot Tools and Reagents Optiblot buffers and stains

InstantBlue® Coomassie蛋白Stain (ISB1L) (ab119211)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (492)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - InstantBlue<sup>&reg;</sup> Coomassie Protein Stain (ISB1L) (ab119211)
  • Western blot - InstantBlue<sup>&reg;</sup> Coomassie Protein Stain (ISB1L) (ab119211)

你可能会感兴趣

一抗
Product image
Anti-CTP synthase/CTPS antibody [EPR8086(B)] - BSA and Azide free (ab211639)
ELISA
Product image
Human FGF19 ELISA Kit (ab230943)

查看更多相关产品

概述

  • 产品名称

    InstantBlue® Coomassie蛋白Stain (ISB1L)
  • 常规说明

    InstantBlue® is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action stains proteins in 15 minutes, while leaving a clear background eliminating the need to fix, wash or destain. Formulated for safe use and easy disposal, it’s ready to use straight out of the bottle and comes in convenient premixed one liter bottles.

    Why InstantBlue® Protein Stain?

    There is no need to wash, fix, microwave or destain gels when using InstantBlue™. Homemade stains and premade solutions require several or all of these extra steps. InstantBlue uses a simple one step protocol.

    The unique patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 minutes. Some premade and traditional homemade Coomassie R-250 protein stains can take three hours or more to fully stain gels, and then require destaining typically overnight.

    Unparalleled sensitivity allows protein levels as low as 5ng per band to be detected (BSA) when staining overnight. This is thanks to InstantBlue’s protein specific staining, which gives a clear background, improving the signal / noise ratio of the stain. Despite the staining power, gels can be left in InstantBlue for weeks and still come out with a crystal clear background, all without the need for destaining or protein stain remover.

    The nontoxic formula is sink disposable and thanks to its methanol free composition will not shrink gels. Other stains require solvent disposal procedures and can require microwaving, which produces dangerous fumes. The effective staining and safe composition of InstantBlue means that there’s no need for microwaving, solvent disposal, or fume hoods.

    InstantBlue will stain at least 40 mini gels per bottle, boasting economical staining with only 25ml per mini gel. As a result, this allows you to use less protein staining solution when compared to 50–100ml of stain per gel for homemade solutions and some premixed solutions.

    Fully compatible with mass spectrometry, gels can be destained using standard procedures. The protein staining solution’s acetic acid free formulation means it will not methylate or acetylate protein during the protein staining protocol.

    Key Benefits

    • Ultra fast staining – Results in 15 minutes or less
    • One step procedure – No washing, fixing, microwaving or destaining
    • High sensitivity – 5ng bands detectable
    • Clear background – High signal / noise ratio
    • Flexible – No overstaining
    • Reliable – Stringent batch to batch consistency
    • Efficient – Only 25ml per gel
    • Safe composition – Nontoxic; no fume hood or solvent disposal required
    • Methanol free – No gel shrinkage or protein methylation
    • Acetic acid free – No protein acetylation
    • Mass spectrometry compatible – Destainable. No residual methylation or acetylation

    This product is manufactured by Expedeon, an Abcam company. Expedeon product code ISB1L was previously called InstantBlue Protein Stain 1 L and is the same as the 1000 ml size of this product.

    Manufactured under patent numbers : US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443

    INSTANTBLUE is a registered trademark of Abcam in the US and an unregistered trademark elsewhere

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at Room Temperature. Store at +4°C.
  • 存储溶液

    Constituents: 0.56% Ethanol, 15.19% Phosphoric acid
  • Concentration information loading...
  • 研究领域

    • Kits/ Lysates/ Other
    • Tools and Reagents
    • Western Blot Tools and Reagents
    • Optiblot buffers and stains
  • 别名

    • Coomassie stain
    • ISB1L
    • SDS-PAGE stain
    • WB stain

图片

  • Western blot - InstantBlue<sup>&reg;</sup> Coomassie Protein Stain (ISB1L) (ab119211)
    Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
  • Western blot - InstantBlue<sup>&reg;</sup> Coomassie Protein Stain (ISB1L) (ab119211)
    Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)This image is courtesy of an anonymous abreview.

    SDS-PAGE image showing molecular weight marker (MW) and His-tag recombinant protein fractions purified in Ni-NTA resin in lanes 1-5 stained with InstantBlue. InstantBlue was easy to handle and use - just pour a small amount onto your gel and within 10 minutes you can see the protein bands! No methanol and glacial acetic acid - no smell!

实验方案

  • Protocol Booklet

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (492)

发表研究结果有使用 ab119211?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab119211 被引用在 492 文献中.

  • Benz PM  et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
  • Pick E Cell-Free NADPH Oxidase Activation Assays: A Triumph of Reductionism. Methods Mol Biol 2087:325-411 (2020). PubMed: 31729001
  • Desrames A  et al. Detergent-free isolation of native red blood cell membrane complexes. Biochim Biophys Acta Biomembr 1862:183126 (2020). PubMed: 31738902
  • Hertz MI  et al. Brugia malayi galectin 2 is a tandem-repeat type galectin capable of binding mammalian polysaccharides. Mol Biochem Parasitol 235:111233 (2020). PubMed: 31738955
  • Forcella M  et al. Neuronal specific and non-specific responses to cadmium possibly involved in neurodegeneration: A toxicogenomics study in a human neuronal cell model. Neurotoxicology 76:162-173 (2020). PubMed: 31738976
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-4 of 4 Abreviews or Q&A

Coomassie stain for protein purification

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
This product works really well at making protein bands visible without the need for destaining, and it works quickly (<15 minutes). I did find, however, that destaining does lower the background, so if you want a crisp image I would recommend destaining.
Lane 1: Protein ladder
Lane 2: Protein before size exclusion chromatography (SEC) column
Lanes 3-12: Elution fractions from SEC column
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 14 2021

Instant Blue Coomassie Protein Stain ( ab119211, abcam) stains gels in 15min.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
The stain works extremely fast and still gives nice dark bands after just 15-20min of staining 2ug of monoclonal antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 05 2021

Protein Purification

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Pretty good! It stains the gel so quickly that i don't have to wait for a long time to see the result. And it works well. Even tiny contamination bands could be seen so I can quickly know how pure my sample is.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Yuntong Wu

Verified customer

提交于 May 12 2021

Amazing product

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
Instant Blue is amazing. I was shocked by how clear the protein bands are, and how easy it is to use. You just need to add the liquid to the gel, leave it for a few minutes, and the bands will develop. No destain step required at all.

This product is very good for the environment as well, as we won’t keep using methanol to make up the destains.

I am very impressed by this product and will keep purchasing it from now on.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 22 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有