Anti-iNOS抗体[K13-A] (ab136918)
Key features and details
- Rabbit monoclonal [K13-A] to iNOS
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
-
产品名称
Anti-iNOS抗体[K13-A]
参阅全部 iNOS 一抗 -
描述
兔单克隆抗体[K13-A] to iNOS -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to a region of Human iNOS.
-
表位
Antibody recognizes the epitope located between Ser1118 - Gly1129 -
阳性对照
- Mouse brain tissue lysate - total protein (ab30151) can be used as a positive control in WB. Raw264.7 whole cell lysate (LPS treated)
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
克隆
单克隆 -
克隆编号
K13-A -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab136918于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/2000. Predicted molecular weight: 131 kDa. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.
|
说明 |
---|
WB
1/2000. Predicted molecular weight: 131 kDa. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. |
靶标
-
功能
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. -
组织特异性
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 18126 Mouse
- Entrez Gene: 24599 Rat
- SwissProt: P29477 Mouse
- SwissProt: Q06518 Rat
- Unigene: 2893 Mouse
- Unigene: 10400 Rat
-
别名
- HEP-NOS antibody
- Hepatocyte NOS antibody
- HEPNOS antibody
see all
图片
-
All lanes : Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
Lane 1 : Raw264.7 Whole Cell Lysate
Lane 2 : Raw264.7 Whole Cell Lysate (LPS stimulated)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anit Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 36 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
-
Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + iNOS Overexpression Lysate (Human) at 0.1 µg
Secondary
Goat anti Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
-
Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 131 kDa
数据表及文件
-
Datasheet download
文献 (18)
ab136918 被引用在 18 文献中.
- Naeem AG et al. Nebivolol elicits a neuroprotective effect in the cuprizone model of multiple sclerosis in mice: emphasis on M1/M2 polarization and inhibition of NLRP3 inflammasome activation. Inflammopharmacology 30:2197-2209 (2022). PubMed: 35948811
- Saeed MEM et al. Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate. Cancer Genomics Proteomics 19:727-739 (2022). PubMed: 36316038
- Fidilio A et al. Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway. Front Pharmacol 13:1075746 (2022). PubMed: 36532726
- Yan J et al. Rhoifolin Ameliorates Osteoarthritis via Regulating Autophagy. Front Pharmacol 12:661072 (2021). PubMed: 34122080
- Kim KJ et al. SR-5, the specific ratio of Korean multi-herbal formula: An evaluation of antiulcerogenic effects on experimentally induced gastric ulcers in mice. Dose Response 19:15593258211044329 (2021). PubMed: 34690616