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Neuroscience Neurotransmission Nitric Oxide NOS

Anti-iNOS抗体(ab3523)

  • Datasheet
  • SDS
Reviews (30)Q&A (33)References (199)

Product price, shipping and contact information

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Immunohistochemistry - Anti-iNOS antibody (ab3523)
  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)
  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)

Key features and details

  • Rabbit polyclonal to iNOS
  • Suitable for: ICC/IF, IHC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-iNOS抗体
    参阅全部 iNOS 一抗
  • 描述

    兔多克隆抗体to iNOS
  • 宿主

    Rabbit
  • 特异性

    This antibody detects iNOS. It does not detect other NOS isoforms. By western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS. By western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells.
  • 经测试应用

    适用于: ICC/IF, IHCmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse iNOS aa 17-31.
    Sequence:

    DLKEEKDINNNVKKT


    Database link: P29477
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • ICC: A549, NIH/3T3 cells. IHC-P: Human heart tissue.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human iNOS protein (ab135010)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

Our Abpromise guarantee covers the use of ab3523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
IHC 1/20.

靶标

  • 功能

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
  • 组织特异性

    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
  • 序列相似性

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Target information above from: UniProt accession P35228 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4843 Human
    • Entrez Gene: 18126 Mouse
    • Entrez Gene: 24599 Rat
    • Omim: 163730 Human
    • SwissProt: P35228 Human
    • SwissProt: P29477 Mouse
    • SwissProt: Q06518 Rat
    • Unigene: 709191 Human
    • Unigene: 2893 Mouse
    • Unigene: 10400 Rat
    see all
  • 别名

    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

图片

  • Immunohistochemistry - Anti-iNOS antibody (ab3523)
    Immunohistochemistry - Anti-iNOS antibody (ab3523)

    Immunohistochemistry analysis of human heart tissue stained for iNOS without (negative control) or using ab3523 at 1/200 dilution overnight at 4°C in a humidified chamber, followed biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.

    To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)
    Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)

    Immunofluorescence analysis of A549 (human lung carcinoma cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    Cells were grown on chamber slides and fixed with formaldehyde prior to staining.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)
    Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)

    Immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    Cells were grown on chamber slides and fixed with formaldehyde prior to staining.

     

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (199)

    发表研究结果有使用 ab3523?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3523 被引用在 199 文献中.

    • Ahmad N  et al. Imperatorin suppresses IL-1ß-induced iNOS expression via inhibiting ERK-MAPK/AP1 signaling in primary human OA chondrocytes. Int Immunopharmacol 85:106612 (2020). PubMed: 32450530
    • Miguel-Jiménez S  et al. Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation? Int J Mol Sci 21:N/A (2020). PubMed: 32197481
    • Zhao JM  et al. Anserine and glucosamine supplementation attenuates the levels of inflammatory markers in rats with rheumatoid arthritis. AMB Express 10:57 (2020). PubMed: 32198574
    • Wang J  et al. Suberoylanilide hydroxamic acid alleviates orthotopic liver transplantation-induced hepatic ischemia-reperfusion injury by regulating the AKT/GSK3ß/NF-?B and AKT/mTOR pathways in rat Kupffer cells. Int J Mol Med 45:1875-1887 (2020). PubMed: 32236599
    • McKelvey KJ  et al. Temporal and spatial modulation of the tumor and systemic immune response in the murine Gl261 glioma model. PLoS One 15:e0226444 (2020). PubMed: 32240177
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 63 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-iNOS antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Permeabilization
    Yes - Triton X 100
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 21 2015

    Question

    mouse liver samples
    sees 3 bands between 120 and 220 kDa
    which one is correct?
    customer will send image for getting advice

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thank you for contacting us.

    Please send me your WB image so I can take a look at the bands you have obtained.

    I look forward to hear back from you.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-iNOS antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Dorsal Root Ganglion)
    Permeabilization
    Yes - triton
    Specification
    Dorsal Root Ganglion
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Xiang Xu

    Verified customer

    提交于 Dec 27 2019

    Western blot abreview for Anti-iNOS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (primary cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12 %)
    Loading amount
    20 µg
    Treatment
    LPS 1 µg/ml
    Specification
    primary cardiomyocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    提交于 Jul 04 2019

    Western blot abreview for Anti-iNOS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HL-1 cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12 %)
    Loading amount
    40 µg
    Treatment
    LPS 1 µg/ml
    Specification
    HL-1 cardiomyocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    提交于 Jul 03 2019

    Western blot abreview for Anti-iNOS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine microglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    1µM LPA for different time points
    Specification
    Primary murine microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Lisha Joshi

    Verified customer

    提交于 Jun 11 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-iNOS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Primary murine microglia)
    Permeabilization
    Yes - Triton X
    Specification
    Primary murine microglia
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Lisha Joshi

    Verified customer

    提交于 Jun 11 2019

    Western blot abreview for Anti-iNOS antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (skin)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Treatment
    UV B (100mJ/cm2)
    Specification
    skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 05 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-iNOS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Axolotl Tissue sections (Arm)
    Permeabilization
    No
    Specification
    Arm
    Blocking step
    DAKO serum free blocking as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
    Fixative
    N/a
    Read More

    Miss. Crystal Jones

    Verified customer

    提交于 Nov 02 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-iNOS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (BV2 or primary microglia)
    Permeabilization
    Yes - TritonX
    Specification
    BV2 or primary microglia
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Joanna Plastira

    Verified customer

    提交于 Oct 12 2018

    1-10 of 63 Abreviews or Q&A

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