Anti-Indoleamine 2,3-dioxygenase抗体[OTI1A3] (ab156787)
Key features and details
- Mouse monoclonal [OTI1A3] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human, African green monkey
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Indoleamine 2,3-dioxygenase抗体[OTI1A3]
参阅全部 Indoleamine 2, 3-dioxygenase 一抗 -
描述
小鼠单克隆抗体[OTI1A3] to Indoleamine 2,3-dioxygenase -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human Indoleamine 2, 3-dioxygenase aa 1-403.
Sequence:MAHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLI ESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVR KVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDV LFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKAL LEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYE GFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMP PAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIV TKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLL KEG
Database link: NP_002155 -
阳性对照
- WB: Wild-type A549 Treated IFN gamma, SK-OV-3, HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA; IHC-P: Human kidney carcinoma, lymph node and lymphoma tissues; ICC/IF: COS7 cells transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography -
克隆
单克隆 -
克隆编号
OTI1A3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/4000. Predicted molecular weight: 45 kDa.
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IHC-P | (1) |
1/150.
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ICC/IF |
1/100.
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说明 |
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WB
1/4000. Predicted molecular weight: 45 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
靶标
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功能
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
通路
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
序列相似性
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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数据库链接
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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别名
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
图片
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All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution
Lane 1 : Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 2 : Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 3 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 4 : IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 5 : SK-OV-3 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab156787 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab156787 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with no signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab156787 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 4000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
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All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [OTI1A3] (ab156787) at 1/4000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 45 kDa -
Immunofluorescent analysis of COS7 cells transiently transfected with pCMV6-ENTRY Indoleamine 2, 3-dioxygenase, labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lymphoma tissue labeling Indoleamine 2, 3-dioxygenase with ab156787 at 1/150 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab156787 被引用在 2 文献中.
- Sun C et al. IDO1 plays a tumor-promoting role via MDM2-mediated suppression of the p53 pathway in diffuse large B-cell lymphoma. Cell Death Dis 13:572 (2022). PubMed: 35760783
- Ye LY et al. Hypoxia-Induced Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma Induces an Immunosuppressive Tumor Microenvironment to Promote Metastasis. Cancer Res 76:818-30 (2016). PubMed: 26837767