Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, U2OS, Jurkat)
Loading amount
20 µg
Specification
HeLa, U2OS, Jurkat
Gel Running Conditions
Reduced Denaturing (5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
2 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Milk, PBS-Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal west femto
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 55, 60 kDa
Positive control
HeLa, Jurkat
Additional data
Additional Notes
Lane order (L-R): HeLa, U2OS, Jurkat
This antibody failed to detect endogenous INCENP (106 kDa) from cells known to express the protein including HeLa (Abcam datasheet) and Jurkat (Cell signalling: A841 - runs at 140 kDa by SDS-PAGE). The reason for the discrepancy in the molecular weights recognised by these two antibodies is unclear.
This antibody failed to detect endogenous INCENP (106 kDa) from cells known to express the protein including HeLa (Abcam datasheet) and Jurkat (Cell signalling: A841 - runs at 140 kDa by SDS-PAGE). The reason for the discrepancy in the molecular weights recognised by these two antibodies is unclear.
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Aug 22 2008