Anti-Importin 13抗体(ab95993)
Key features and details
- Rabbit polyclonal to Importin 13
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Importin 13抗体
参阅全部 Importin 13 一抗 -
描述
兔多克隆抗体to Importin 13 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide containing a sequence corresponding to a region within amino acids 901-963 (SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT ADY) of Human Importin 13 (NP_055467).
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阳性对照
- HeLa whole cell lysate (ab150035); HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95993于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 108 kDa.
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IP |
Use at 10-1 µg/mg of lysate.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 108 kDa. |
IP
Use at 10-1 µg/mg of lysate. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13. -
组织特异性
Expressed in fetal brain, heart, intestine and kidney. -
序列相似性
Belongs to the importin beta family.
Contains 14 HEAT repeats.
Contains 1 importin N-terminal domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 523399 Cow
- Entrez Gene: 9670 Human
- Entrez Gene: 230673 Mouse
- Entrez Gene: 116458 Rat
- GenBank: NM_014652 Human
- Omim: 610411 Human
- SwissProt: A7YWD2 Cow
- SwissProt: O94829 Human
see all -
别名
- Imp 13 antibody
- Imp13 antibody
- Importin-13 antibody
see all
图片
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All lanes : Anti-Importin 13 antibody (ab95993)
Lane 1 : Non-transfected lysate
Lane 2 : IPO13 transfected lysate
Predicted band size: 108 kDa -
Importin 13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 µg of ab95993. The precipitated Importin 13 was detected with ab95993 at 1/1000 dilution.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab95993 at 1/200 dilution. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab95993 被引用在 2 文献中.
- Kalita J et al. On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions. J Cell Sci 134:N/A (2021). PubMed: 33912945
- Xue J et al. Forkhead Box M1 Is Essential for Nuclear Localization of Glioma-associated Oncogene Homolog 1 in Glioblastoma Multiforme Cells by Promoting Importin-7 Expression. J Biol Chem 290:18662-70 (2015). PubMed: 26085085