Key features and details
- Rabbit polyclonal to ILF3/NF90
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
参阅全部 ILF3/NF90 一抗
经测试应用适用于: WB, IPmore details
预测可用于: Rhesus monkey, Opossum
Synthetic peptide corresponding to Human ILF3/NF90 aa 652-702.
Database link: NP_004507.2
- 购买相配的WB阳性对照Recombinant Human ILF3/NF90 protein
- HeLa, Jurkat and 293T whole cell lysates.
This product was previously labelled as ILF3
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab131004 is affinity purified using an epitope specific to ILF3/NF90 immobilized on solid support.
Our Abpromise guarantee covers the use of ab131004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 95 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
功能May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
序列相似性Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 DZF domain.
翻译后修饰Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
Methylated by protein arginine N-methyltransferase 1.
Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
细胞定位Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- CBTF antibody
- Double stranded RNA binding protein 76 antibody
- Double-stranded RNA-binding protein 76 antibody
All lanes : Anti-ILF3/NF90 antibody (ab131004) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 10 seconds
Detection of ILF3/NF90 by Western Blot of Immunprecipitate.
ab131004 at 1µg/ml staining ILF3/NF90 in HeLa whole cell lysate immunoprecipitated using ab131004 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab131004 被引用在 2 文献中.
- Lin J et al. An NF90/long noncoding RNA-LET/miR-548k feedback amplification loop controls esophageal squamous cell carcinoma progression. J Cancer 10:5139-5152 (2019). PubMed: 31602267
- Jiang Z et al. LincIN, a novel NF90-binding long non-coding RNA, is overexpressed in advanced breast tumors and involved in metastasis. Breast Cancer Res 19:62 (2017). PubMed: 28558830