Anti-IL-8抗体(ab106350)
Key features and details
- Rabbit polyclonal to IL-8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-8抗体
参阅全部 IL-8 一抗 -
描述
兔多克隆抗体to IL-8 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IL-8 aa 77-95 (C terminal).
Sequence:CLDPKENWVQRVVEKFLKR
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阳性对照
- IHC-P: Human appedicitis tissue. WB: Recombinant Human IL8 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106350于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 11 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 11 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
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别名
- (Ala-IL-8)77 antibody
- (Ser-IL-8)72 antibody
- 9E3 antibody
see all
图片
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ab106350, at 1µg/ml, staining IL8 in paraffin-embedded human appedicitis tissue by Immunohistochemistry.
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All lanes : Anti-IL-8 antibody (ab106350) at 1 µg/ml
Lane 1 : Recombinant Human IL8 protein at 20ng
Lane 2 : Recombinant Human IL8 protein at 10ng
Lane 3 : Recombinant Human IL8 protein at 5ng
Lane 4 : Raji whole cell lysate
Lane 5 : MK (55kD)
Predicted band size: 11 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab106350 被引用在 16 文献中.
- Jiang H et al. Targeting IL8 as a sequential therapy strategy to overcome chemotherapy resistance in advanced gastric cancer. Cell Death Discov 8:235 (2022). PubMed: 35487914
- Allouch A et al. CDKN1A is a target for phagocytosis-mediated cellular immunotherapy in acute leukemia. Nat Commun 13:6739 (2022). PubMed: 36347876
- Kai K et al. Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production. Int J Mol Sci 22:N/A (2021). PubMed: 34502382
- Lee S et al. Virus-induced senescence is a driver and therapeutic target in COVID-19. Nature 599:283-289 (2021). PubMed: 34517409
- Merz V et al. Plasma IL8 Is a Biomarker for TAK1 Activation and Predicts Resistance to Nanoliposomal Irinotecan in Patients with Gemcitabine-Refractory Pancreatic Cancer. Clin Cancer Res 26:4661-4669 (2020). PubMed: 32532788