The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 15, 17 kDa (predicted molecular weight: 18 kDa).
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes.
The predicted Mw for is 18-kDa. However, the sequence of IL17F protein contains a 30-residue signal sequence and a potential glycosylation site, which might explain the difference between the predicted molecular weight and that observed here. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.