Anti-IL1 alpha抗体[AS5] (ab17281)

概述

  • 产品名称
    Anti-IL1 alpha抗体[AS5]
    参阅全部 IL1 alpha 一抗
  • 描述
    小鼠单克隆抗体[AS5] to IL1 alpha
  • 宿主
    Mouse
  • 特异性
    Recognizes human IL-1 alpha. Does not recognize mouse IL-1 alpha, human IL-1 beta or a panel of other human cytokines.
  • 经测试应用
    适用于: IHC-P, IP, ELISA, Neutralising, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant IL1 alpha protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab17281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200. (see Abreview)
IP Use at an assay dependent concentration. This antibody has been used to immunoprecipitate >90% human IL-1 (in solution using a double-antibody method).
ELISA Use a concentration of 2 µg/ml. This antibody has been used as a solution phase antibody in a two site ELISA with biotin conjugated IL-1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human IL-1 alpha in human serum is typically >85% in the ELISA.
Neutralising Use a concentration of 25 mg/ml. This antibody has been used to neutralize >95% of the biological activity of human IL-1 at 0.5 mg/ml.
IHC (PFA fixed) Use a concentration of 1 - 10 µg/ml. This antibody has been used to stain human IL-1 alpha expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4oC plus 0.1% saponin 20 minutes at RT.

靶标

  • 功能
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性
    Belongs to the IL-1 family.
  • 结构域
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all

图片

  • ab17281 staining IL1 alpah in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes at 21°C. A undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Zhang X  et al. Expression of TP53 and IL-1a in unicystic ameloblastoma predicts the efficacy of marsupialization treatment. Medicine (Baltimore) 97:e9795 (2018). Read more (PubMed: 29419674) »
  • Zhang X  et al. H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis. Nat Commun 8:14799 (2017). Read more (PubMed: 28300060) »

See all 2 Publications for this product

客户评价及客户问答

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH 9.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册