The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. (see Abreview)
Use at an assay dependent concentration. This antibody has been used to immunoprecipitate >90% human IL-1 (in solution using a double-antibody method).
Use a concentration of 2 µg/ml. This antibody has been used as a solution phase antibody in a two site ELISA with biotin conjugated IL-1 alpha antibody. Typically the ELISA has a working range of 0-10000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of human IL-1 alpha in human serum is typically >85% in the ELISA.
Use a concentration of 25 mg/ml. This antibody has been used to neutralize >95% of the biological activity of human IL-1 at 0.5 mg/ml.
IHC (PFA fixed)
Use a concentration of 1 - 10 µg/ml. This antibody has been used to stain human IL-1 alpha expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4oC plus 0.1% saponin 20 minutes at RT.
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Belongs to the IL-1 family.
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL1 alpha antibody [AS5] (ab17281)Image courtesy of an anonymous Abreview.
ab17281 staining IL1 alpah in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 30 minutes at 21°C followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes at 21°C. A undiluted HRP-conjugated goat polyclonal was used as secondary antibody.