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Cardiovascular Angiogenesis Cytokines Interleukin

Anti-IL-8抗体(ab7747)

  • Datasheet
Reviews (3)Q&A (11)References (62)

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ELISA - Anti-IL-8 antibody (ab7747)

    Key features and details

    • Rabbit polyclonal to IL-8
    • Suitable for: ELISA
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-IL-8抗体
      参阅全部 IL-8 一抗
    • 描述

      兔多克隆抗体to IL-8
    • 宿主

      Rabbit
    • 特异性

      Please be aware that this product has low homology with the mouse and rat sequence of IL8 (Rat, 46%; Mouse 45%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
    • 经测试应用

      适用于: ELISAmore details
    • 种属反应性

      与反应: Human
      预测可用于: Cynomolgus monkey, Rhesus monkey
    • 免疫原

      Recombinant full length protein corresponding to IL-8.

    • 阳性对照

      • 购买相配的WB阳性对照Recombinant human IL-8 protein
      • ELISA: Human IL-8.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      Constituents: 0.1% BSA, PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human IL-8 protein (Active) (ab259397)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7747于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ELISA
    1/50.
    说明
    ELISA
    1/50.

    靶标

    • 功能

      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • 序列相似性

      Belongs to the intercrine alpha (chemokine CxC) family.
    • 翻译后修饰

      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • 细胞定位

      Secreted.
    • Target information above from: UniProt accession P10145 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 3576 Human
      • Omim: 146930 Human
      • SwissProt: P10145 Human
      • Unigene: 624 Human
      • 别名

        • (Ala-IL-8)77 antibody
        • (Ser-IL-8)72 antibody
        • 9E3 antibody
        • Beta thromboglobulin like protein antibody
        • C-X-C motif chemokine 8 antibody
        • CEF-4 antibody
        • chemokine, CXC motif, ligand 8 antibody
        • CXCL8 antibody
        • Emoctakin antibody
        • GCP-1 antibody
        • GCP/IL-8 protein I antibody
        • GCP/IL-8 protein II antibody
        • GCP/IL-8 protein III antibody
        • GCP/IL-8 protein IV antibody
        • GCP/IL-8 protein V antibody
        • GCP/IL-8 protein VI antibody
        • GCP1 antibody
        • Granulocyte chemotactic protein 1 antibody
        • IL-8 antibody
        • IL-8(1-77) antibody
        • IL-8(9-77) antibody
        • IL8 antibody
        • IL8/NAP1 form I antibody
        • IL8/NAP1 form II antibody
        • IL8/NAP1 form III antibody
        • IL8/NAP1 form IV antibody
        • IL8/NAP1 form V antibody
        • IL8/NAP1 form VI antibody
        • IL8_HUMAN antibody
        • Inteleukin 8 antibody
        • LECT antibody
        • LUCT antibody
        • Lymphocyte-derived neutrophil-activating factor antibody
        • LYNAP antibody
        • MDNCF antibody
        • MDNCF-b antibody
        • MDNCF-c antibody
        • MONAP antibody
        • Monocyte derived neutrophil activating peptide antibody
        • Monocyte derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil chemotactic factor antibody
        • Monocyte-derived neutrophil-activating peptide antibody
        • NAF antibody
        • NAP 1 antibody
        • NAP-1 antibody
        • NAP1 antibody
        • Neutrophil activating peptide 1 antibody
        • Neutrophil activating protein 1 antibody
        • Neutrophil-activating factor antibody
        • Neutrophil-activating protein 1 antibody
        • Protein 3 10C antibody
        • Protein 3-10C antibody
        • SCYB 8 antibody
        • SCYB8 antibody
        • Small inducible cytokine subfamily B member 8 antibody
        • T cell chemotactic factor antibody
        • T-cell chemotactic factor antibody
        • TSG 1 antibody
        • TSG1 antibody
        see all

      图片

      • ELISA - Anti-IL-8 antibody (ab7747)
        ELISA - Anti-IL-8 antibody (ab7747)

        Specificity test of ab7747.

        ab7747 was biotinylated and used as a detection antibody in different concentrations.

        Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

      实验方案

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (62)

      发表研究结果有使用 ab7747?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab7747 被引用在 62 文献中.

      • Abbasi M  et al. Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments. Cureus 13:e13499 (2021). PubMed: 33777584
      • Cao R  et al. Nell-1 attenuates lipopolysaccharide-induced inflammation in human dental pulp cells. J Mol Histol 52:671-680 (2021). PubMed: 33905072
      • Wang Q  et al. Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity. Bosn J Basic Med Sci 21:81-92 (2021). PubMed: 32343942
      • Azevedo MLV  et al. Lung Neutrophilic Recruitment and IL-8/IL-17A Tissue Expression in COVID-19. Front Immunol 12:656350 (2021). PubMed: 33868301
      • Sarvestani SK  et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun 12:262 (2021). PubMed: 33431859
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 14 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL8 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Cynomolgus Monkey Tissue sections (Macaque Retina)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
      Permeabilization
      No
      Specification
      Macaque Retina
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 01 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IL8 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Muller Cell Line MIO-M1)
      Permeabilization
      Yes - 0.1% TritonX-100
      Specification
      Muller Cell Line MIO-M1
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Aug 01 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL8 antibody

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (HNSCC Tissue)
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Specification
      HNSCC Tissue
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Dr. Biswanath Majumder

      Verified customer

      提交于 Jul 01 2015

      Question

      Sehr geehrte Damen und Herren,
      in unserer AG besteht Interesse an Ihrem Anti-Interleukin-8 Ab (ab7747). Können Sie mir
      ein Angebot für diesen Antikörper (plus Isotype Kontrolle) zukommen lassen? Ist dieser Antikörper
      für die Durchflusszytometrie geeignet? (data sheet macht dazu leider keine Angaben! nurIHC-P, WB, IP & ELISA)
      Für eine Antwort wäre ich Ihnen sehr dankbar und verbleibe
      mit freundlichen Grüßen

      Read More

      Abcam community

      Verified customer

      Asked on Oct 25 2012

      Answer

      Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

      Unseres Wissens nach wurde ab7747 bisher noch nicht in Durchflusszytometrie getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab7747 abdecken, und gegen eine erneute Bestellung weitere Produkte von uns verrechnet werden.

      Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

      1.) Bestätigen Sie mir bitte, dass Sie ab7747 in Durchflusszytometrie testen möchten.

      2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

      3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

      4.) Testen Sie den Antikörper in Durchflusszytometrie.

      5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
      Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
      https://www.abcam.com/abreviews

      6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie könnenweitere Produkte zu dem gleichen Preis wie ab7747 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab7747 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

      Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

      Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
      https://www.abcam.com/collaborationdiscount

      Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

      Read More

      Abcam Scientific Support

      回复于 Oct 25 2012

      Question

      I am a Ph.D. student doing research on the effects of obesity on the placental development. Unfortunately the antibodies of interest have not been tested in the uterus/placenta. I am writing to see if your company would willing to give us a small sample of the anti-IL-8 catalog # ab7747 and anti MCP-1 catalog # ab7202 to do a test by immunohistochemistry on paraffin embedded samples of the rat uterus/placenta. I would be willing to share my results to help promote this product.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 20 2012

      Answer

      Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

      We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

      Read More

      Abcam Scientific Support

      回复于 Aug 20 2012

      Question

      Thank you very much for your fast response, however I have another question:
      Is it alwaysnecessaryto use a secondaryantibody, I mean can I do it with primary antibody alone
      and if not, what is the best recommended secondary antibody, because I saw related secondary antibody for electron microscopy application in the product page, and I am using IHC-Pr
      I am asking so for my personal preference to use primary antibody alone in IHC staining without secondary antibody and I hope that would be possible,
      thank you very much indeed
      Sincerely

      Read More

      Abcam community

      Verified customer

      Asked on Jun 15 2012

      Answer

      Thank you for your reply.

      In theory it is possible to perform IHC-P with a directly conjugated antibody. This will also mean that the amplification that is achieve by adding the secondary is lost.

      So my advise would be to only do it for abundant antigens.

      ab7747 is not conjugated to HRP and therefore will need a secondary antibody that is conjugated.

      I can recommend to use one of these:

      ab98485

      https://www.abcam.com/Goat-Fab2-Rabbit-IgG-Fc-HRP-pre-adsorbed-ab98485.html (or use the following: https://www.abcam.com/Goat-Fab2-Rabbit-IgG-Fc-HRP-pre-adsorbed-ab98485.html).

      ab97085

      https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-preadsorbed-ab97085.html (or use the following: https://www.abcam.com/Donkey-Rabbit-IgG-HL-HRP-preadsorbed-ab97085.html).

      We also offer EasyLink kits that can be used to conjugate this antibody directly to HRP (ab102892).

      https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-10g-HRP-ab102892.html (or use the following: https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-10g-HRP-ab102892.html).

      It would not be 100% optimal in this case since the antibody is only purified as IgG fraction.

      I hope this information is helpful and wish you a good weekend.

      Read More

      Abcam Scientific Support

      回复于 Jun 15 2012

      Question

      Dear Sir,
      have a nice day

      we purchased Anti-IL-8 antibody (ab7747) for immunohistochemistry inparaffin sections IHC-P, it wasn't mentioned what is the diluation that you recommend, you provided 6 references for the product but I have problem with access and publisher asked for money to reveal full text of articles (non of them is free in pubmed).

      here I would like to request you to provide us with the recommended titre (dilution of antibody) either you recommend or from the 6 references you provided for the product

      thank you for much indeed

      yours,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 15 2012

      Answer

      Thank you for your inquiry.

      I am happy to confirm that the recommended protocol in IHC is the following:

      Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

      Antibody incubation: 120 min, 20C 1:500

      (http://www.ncbi.nlm.nih.gov/pubmed/17208399?dopt=Abstract)

      I hope this information is helpful and wish you good luck with your experiments.

      Read More

      Abcam Scientific Support

      回复于 Jun 15 2012

      Question

      No staining in IHC with another vial of ab7747.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 20 2012

      Answer

      Thank you for your call today and for letting us know about the trouble with ab7747.

      As we discussed, I'm sending a free of charge vial of ab18672 on the order *** which should arrive tomorrow.

      Please keep me updated about the results using this new antibody. I look forward to hearing from you, and let me know if you have any questions or if there is anything else that we can do for you.

      Read More

      Abcam Scientific Support

      回复于 Mar 20 2012

      Question

      Phoned because this product did not work in IHC-P.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 02 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      回复于 Feb 02 2012

      Question

      Sorry about that - here it is. Primary antibody: ab7747 Secondary antibody: Biotinylated goat anti-rabbit IgG [a competitor] Chromogen: AEC Procedure: 1. Place slides on hot plate, 65 C for 30 minutes, deparaffinize in xylenes 4 min x 2, hydrate through alcohol –100% 2 min x 2, and 95% alcohol 2min x 2, 80% alcohol 2min x 1, then wash with PBS 3 min x2. 2. Perform antigen retrieval: Dako target retrieval buffer. Place slides in coplin jar filled with 1X antigen retrieval buff (DAKO). Pre-heat buffer for 20 minutes in steamer. Add tissue for 20 min, then leave in steamer to cool down for 30 min. 3. Draw a hydrophobic circle around the tissue with a pap pen. Add a drop of PBS and leave in humidity chamber. 4. Block endogenouse peroxides with 3% H2O2 in PBS for 12 min. 5. Wash with PBS 3min x 3. 6. Incubate with protein clocking solution (5% normal horse serum + 1% normal goat serum in PBS) 20 min. 7. Remove excess protein block soln, and add primary antibody in protein block solution (see antibody dilution schedule) and incubate overnight at 4 C. 8. Day 2, wash with PBS 3min x 3. 9. Add protein block solution and incubate for 10 min. 10. Remove excess protein block soln, add secondary antibody: biotinylated goat anti-rabbit IgG (Biocare Medical) 30 min, 11. Wash with PBS 3min x3 12. Strepavidin conjugated with horseradish peroxidase (Dako) in PBS (1:300) for 30 min. 13. Wash with PBS 3min x 3, followed by a brief rinse in dH2O with brij (1 drop of brij in 50 ml of PBS) 14. Add chromogen (AEC) and incubate for 2-5 min at RT. Check the reaction microscopically to determine the end point. 15. Wash with dH2O and dry on heat 65 C for 10-20 min, then mount with universal or Permont (Fisher) mounting solution. Leave slides overnight for a complete dry.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 08 2006

      Answer

      Thank you for your protocol, I have looked at it in details and I would like to suggest trying the following changes to see if you start having a cytoplasmic staining rather than a nuclear staining. -check you are not overfixing (or underfixing) the tissue, different fixation times could be tested to see if this may be a problem -try a different antigen retrieval method, such as enzymatic. This way if the citrate buffer antigen retrieval method is damaging the epitope (and other proteins) you may see a difference with a different antigen retrieval step -check with a no primary control that the staining is not due to the secondary or other further steps in the staining protocol -try blocking peroxidases with only 0.3% 10-20min, too much peroxidase can damage the tissue. -remove the horse serum and use only 10% normal goat serum to block the tissue (just in case the serum is giving the problem) I think in general your protocol is very good and hope that the above suggestions will help. If you want to be certain of the staining I would recommend to run another IL8 antibody along the sister sections to see if it gives you the same staining pattern. As ab7747 has not been tested in IHC it is hard to be sure the problem is not that the antibody does not work in IHC.

      Read More

      Abcam Scientific Support

      回复于 Feb 09 2006

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      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
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