Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary murine microglia)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Treatment
1µM LPA for different time points
Specification
Primary murine microglia
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% BSA
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 32 kDa
Additional data
Additional Notes
Even though the Ab is not predicted for mouse, it worked quite well with our samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Lisha Joshi
Verified customer
提交于 Jun 11 2019