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Immunology Innate Immunity Cytokines Interleukins

Anti-IL-6抗体(ab6672)

  • Datasheet
  • SDS
Reviews (44)Q&A (38)References (420)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
  • Western blot - Anti-IL-6 antibody (ab6672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
  • Western blot - Anti-IL-6 antibody (ab6672)

Key features and details

  • Rabbit polyclonal to IL-6
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-IL-6抗体
    参阅全部 IL-6 一抗
  • 描述

    兔多克隆抗体to IL-6
  • 宿主

    Rabbit
  • 特异性

    ab6672 detects a band at 25 kDa in human lung tissue lysate and mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. Please be aware that this product has low homology with the mouse and rat sequence of IL6 (Rat, 40%; Mouse 41%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Pig
  • 免疫原

    Recombinant full length protein corresponding to Human IL-6. Produced in E.coli.
    Database link: P05231

  • 阳性对照

    • 购买相配的WB阳性对照Recombinant Human IL-6 protein
    • WB: Recombinant Human IL6 protein (ab101044), lysate of 2 x 10<6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS).
  • 常规说明

    IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Metabolic disorders

相关产品

  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human IL-6 protein (ab101044)
  • Recombinant Protein

    • Recombinant human IL-6 protein (Active) (ab259381)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • TRAP-6, PAR1 agonist (ab120801)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6672于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (14)
1/500 - 1/2000.
IHC-P (15)
1/400 - 1/800.
说明
WB
1/500 - 1/2000.
IHC-P
1/400 - 1/800.

靶标

  • 功能

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 疾病相关

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 序列相似性

    Belongs to the IL-6 superfamily.
  • 翻译后修饰

    N- and O-glycosylated.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 3569 Human
    • Omim: 147620 Human
    • SwissProt: P05231 Human
    • Unigene: 654458 Human
    • 别名

      • Interleukin BSF 2 antibody
      • B cell differentiation factor antibody
      • B cell stimulatory factor 2 antibody
      • B-cell stimulatory factor 2 antibody
      • BSF 2 antibody
      • BSF-2 antibody
      • BSF2 antibody
      • CDF antibody
      • CTL differentiation factor antibody
      • Cytotoxic T cell differentiation factor antibody
      • Hepatocyte stimulating factor antibody
      • Hepatocyte stimulatory factor antibody
      • HGF antibody
      • HSF antibody
      • Hybridoma growth factor antibody
      • Hybridoma growth factor Interferon beta-2 antibody
      • Hybridoma plasmacytoma growth factor antibody
      • IFN-beta-2 antibody
      • IFNB2 antibody
      • IL 6 antibody
      • IL-6 antibody
      • IL6 antibody
      • IL6_HUMAN antibody
      • Interferon beta 2 antibody
      • Interferon beta-2 antibody
      • Interleukin 6 antibody
      • Interleukin 6 (interferon beta 2) antibody
      • Interleukin BSF 2 antibody
      • Interleukin-6 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)

      IHC image of IL6 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6672, 1/400, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-IL-6 antibody (ab6672)
      Western blot - Anti-IL-6 antibody (ab6672)
      All lanes : Anti-IL-6 antibody (ab6672) at 1/500 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human lung tissue lysate
      Lane 3 : Mouse spleen tissue lysate
      Lane 4 : Mouse lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Additional bands at: 25 kDa, 55 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.



      Tissue lysates were denatured for 10-15 minutes at 90ºC. ab6672 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.

      ab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)Lee EJ et al. Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells. PLoS One 11:e0146463 (2016).

      IL6 (Red) and IL-12 (Green) were measured at 1, 3, and 7 days after 10 Gy irradiation of HCa-1 tumors to determine whether irradiation regulates IL-12 and IL6 expression in tumours. ab6672 was used to stain IL6 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-IL-6 antibody (ab6672)
      Western blot - Anti-IL-6 antibody (ab6672)
      Anti-IL-6 antibody (ab6672) at 1/500 dilution + recombinant human IL-6

      Secondary
      conjugated anti-Rabbit IgG at 1/40000 dilution

      Developed using the ECL technique.

      Observed band size: 21 kDa why is the actual band size different from the predicted?



      4-20% Tris-Glycine gel.

      The membrane was blocked for 30 minutes with 1% BSA-TBST.

    实验方案

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (420)

    发表研究结果有使用 ab6672?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6672 被引用在 420 文献中.

    • Sun X  et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
    • Huehnchen P  et al. Blockade of IL-6 signaling prevents paclitaxel-induced neuropathy in C57Bl/6 mice. Cell Death Dis 11:45 (2020). PubMed: 31969555
    • Nam JE  et al. Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu. Life Sci 254:117742 (2020). PubMed: 32360619
    • Lambert K  et al. Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice. Food Chem Toxicol 141:111403 (2020). PubMed: 32387306
    • Koppinger MP  et al. Lactobacillus reuteri attenuates cardiac injury without lowering cholesterol in low-density lipoprotein receptor-deficient mice fed standard chow. Am J Physiol Heart Circ Physiol 319:H32-H41 (2020). PubMed: 32412785
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 82 Abreviews or Q&A

    Question

    Oops...I guess I forgot to attach it. Yes, it is for ab6672. My data sheet says 80 mg/ml. Most reviewers of this antibody on your website have used a 1/200 dilution on formalin fixed, paraffin embedded tissue. Wonder whether there were any problems with nonspecific binding at such a high antibody concentration.

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    Thank you for your reply.

    For ab6672, it is supplied as a whole antiserum, this means that it is not a purified antibody. The concentration range of 80-85mg/ml, represents the total protein concentration, the actual concentration of the IL6 antibody present will be significantly lower (usually between 5-10% of the total concentration). Therefore the concentrations that reviewers have specified they used would be appropriate.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 May 01 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Kupffer Cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Kupffer Cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 21 2020

    Western blot abreview for Anti-IL-6 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HepG2 cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    25 µg
    Specification
    HepG2 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 27 2020

    Western blot abreview for Anti-IL-6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (primary cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20 %)
    Loading amount
    20 µg
    Treatment
    1 µg/ml LPS for 8 h
    Specification
    primary cardiomyocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    提交于 Aug 21 2019

    Western blot abreview for Anti-IL-6 antibody

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary murine microglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Treatment
    1µM LPA for different time points
    Specification
    Primary murine microglia
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Lisha Joshi

    Verified customer

    提交于 Jun 11 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse tumor tissue)
    Permeabilization
    Yes - PBST(0.5% v/v triton X-100, sigma)
    Specification
    mouse tumor tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 29 2019

    Western blot abreview for Anti-IL-6 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (RBL basophils)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine gel)
    Loading amount
    50 µg
    Treatment
    LPS 10µg/mL
    Specification
    RBL basophils
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Joanna Plastira

    Verified customer

    提交于 Oct 29 2018

    Western blot abreview for Anti-IL-6 antibody

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MG-63 osteosarcoma cell line)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine gel)
    Loading amount
    50 µg
    Treatment
    LPS 10µg/mL
    Specification
    MG-63 osteosarcoma cell line
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Joanna Plastira

    Verified customer

    提交于 Oct 29 2018

    Western blot abreview for Anti-IL-6 antibody

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HL-1 mouse cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    30 µg
    Treatment
    LPS 10µg/mL
    Specification
    HL-1 mouse cardiomyocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Joanna Plastira

    Verified customer

    提交于 Oct 29 2018

    Immunohistochemistry free floating abreview for Anti-IL6 antibody

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    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Brain Tissue - Hippocampus)
    Specification
    Brain Tissue - Hippocampus
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    Abcam user community

    Verified customer

    提交于 Sep 24 2018

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