Anti-IL-4抗体(ab9622)
Key features and details
- Rabbit polyclonal to IL-4
- Suitable for: ELISA, WB, Sandwich ELISA, Neutralising
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-4抗体
参阅全部 IL-4 一抗 -
描述
兔多克隆抗体to IL-4 -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, Sandwich ELISA, Neutralisingmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-4. Highly pure (>98%) recombinant hIL-4 (human Interleukin-4)
Database link: P05112 -
阳性对照
- Recombinant human IL4 protein (ab9623) can be used as a positive control in WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
To detect hIL-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as Detection antibody with recommended pair. |
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. |
说明 |
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ELISA
Use at an assay dependent concentration. To detect hIL-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. |
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. |
靶标
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功能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
疾病相关
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
序列相似性
Belongs to the IL-4/IL-13 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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别名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab9622 被引用在 33 文献中.
- Virgens AR et al. Perivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinone. Exp Dermatol 31:191-201 (2022). PubMed: 34358352
- Kurkipuro J et al. Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds. PLoS One 17:e0264775 (2022). PubMed: 35226700
- Baba T et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration. Elife 9:N/A (2020). PubMed: 32366355
- Yi P et al. Impact of chitosan membrane culture on the expression of pro- and anti-inflammatory cytokines in mesenchymal stem cells. Exp Ther Med 20:3695-3702 (2020). PubMed: 32855721
- Dey P et al. Oncogenic KRAS-Driven Metabolic Reprogramming in Pancreatic Cancer Cells Utilizes Cytokines from the Tumor Microenvironment. Cancer Discov 10:608-625 (2020). PubMed: 32046984