存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab116280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).|
组织特异性Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
序列相似性Belongs to the IL-1 family.
- Information by UniProt
- 2310043N20Rik antibody
- Family of interleukin 1 eta antibody
- FIL1 (ETA) antibody
All lanes : Anti-IL-36 beta / IL-1F8 antibody (ab116280) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Tonsil (Human) Tissue Lysate
Lane 2 : Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
All lanes : Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa (possible non-specific binding)
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab116280 has not yet been referenced specifically in any publications.